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Before you measure with it you set both sides of the balance equal to zero so then when you measure they will either equal the same or balance out.
Yes - otherwise it will give a false reading.
You will need an analytical balance to weigh the 400 mg of tetracycline, assuming it is in solid form to start with. Put a piece of weighing paper onto the balance, and tare it to zero. Spoon the tetracycline onto the paper until the balance reads 400 mg. Pour the tetracycline into a gallon of water, and stir until it dissolves.
The reading could be too high or too low.
which the body is not balanced