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Q: Why should the standard plate count be between 30 and 300 colonies?
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How could the pour plate procedure be used for counting the number of bacteria in a sample?

It is recommended that plates that should be counted should be between 30 and 300 McCance and Harrigan (1992). When more that 300 colonies you cant count them with great accuracy and represented with TNC ( too numerous to count). If colonies are less that 30 do you conclude that there was no bacteria in a product or food, if counting less than 20 colonies is unrealistic. Some standards are zero tolerant where even one colony means alot. So IDF (19991/1992) came up with a formula which takes in account the plates with/ even less than 30 colonies. The formula takes into account all sums of colonies obtained on the pour plates to come up with total cfus


How do you determine the number of organisms in a water sample if your dilution plate has presence of spreading colonies?

To determine the number of organisms in a water sample when spreading colonies are present on a dilution plate, you would count the number of colonies on a plate with a countable amount (usually between 30-300 colonies). Then, you would multiply this count by the dilution factor used and adjust for the volume plated to calculate the total number of organisms in the original water sample. Keep in mind that any spreading colonies need to be accounted for in this calculation.


What is affect of spores on tpc count?

Spores do not contribute to the total plate count (TPC) as they do not metabolize on standard culture media used for TPC determination. Only viable bacteria that can grow and form colonies will affect the TPC count. Spores may appear on plates as the result of sporulation by certain bacteria, but they are not included in the TPC calculation.


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Why is the total viable count based on 30-300 colonies of microorganisms?

The range of 30-300 colonies is used because it provides a statistically significant sample size for counting colonies and estimating the number of viable microorganisms in a given sample. If there are too few colonies, the count may not be representative of the actual microbial population, and if there are too many colonies, it can be difficult to accurately count and differentiate individual colonies.


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What if your instructor asks you to determine the number of organisms in a water sample observation of your dilution plates reveals?

I think you would eliminate plate counts that are not between 30-300 colonies. <30, because its too few to count, >300 too numerous to count.


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Can you grow all type of microorganism in standard plate count?

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