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Differential spectrophotometry is a spectrophotometric analytical technique in which a solution of the sample's major component is placed in the reference cell and the recorded spectrum represents the difference between the sample cell and the reference cell...basically it uses major component of system as reference and NOT solvent ..for example if a enzyme ligand system is to be assayed ..enzyme + solvent is reference and enzyme + ligand + solvent is test sample..its for quantitative detection.

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6mo ago

Spectrophotometry measures the amount of light absorbed or transmitted by a sample across a range of wavelengths, providing information on the sample's concentration and chemical structure. Differential spectrophotometry compares the absorption of light between two samples or two different conditions of the same sample, highlighting differences in concentration or composition.

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Q: Difference between spectrophotometry and differential spectrophotometry?
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