answersLogoWhite

0

Differential spectrophotometry is a spectrophotometric analytical technique in which a solution of the sample's major component is placed in the reference cell and the recorded spectrum represents the difference between the sample cell and the reference cell...basically it uses major component of system as reference and NOT solvent ..for example if a enzyme ligand system is to be assayed ..enzyme + solvent is reference and enzyme + ligand + solvent is test sample..its for quantitative detection.

User Avatar

Wiki User

8y ago

Still curious? Ask our experts.

Chat with our AI personalities

JudyJudy
Simplicity is my specialty.
Chat with Judy
ProfessorProfessor
I will give you the most educated answer.
Chat with Professor
JordanJordan
Looking for a career mentor? I've seen my fair share of shake-ups.
Chat with Jordan
More answers

Spectrophotometry measures the amount of light absorbed or transmitted by a sample across a range of wavelengths, providing information on the sample's concentration and chemical structure. Differential spectrophotometry compares the absorption of light between two samples or two different conditions of the same sample, highlighting differences in concentration or composition.

User Avatar

AnswerBot

11mo ago
User Avatar

Add your answer:

Earn +20 pts
Q: Difference between spectrophotometry and differential spectrophotometry?
Write your answer...
Submit
Still have questions?
magnify glass
imp
Continue Learning about Natural Sciences

How do you calculate Orifice plate differential pressure?

To calculate the orifice plate differential pressure, you can use the Bernoulli's equation or the ISO 5167 standard equation. Measure the pressure upstream and downstream of the orifice plate using pressure gauges, then find the difference between these two pressures to determine the differential pressure across the orifice plate.


Differential CT on live and neutral terminal?

A differential CT measures the difference in current between two terminals. When placed on the live and neutral terminals, the CT will detect any imbalance in current flow between them, which can indicate faults or leakage to ground in the system. This can be used for protective relaying or ground fault detection applications.


What is Differential isolation?

Differential isolation refers to the ability to isolate or separate certain components or signals from others in a system. This is often done to prevent interference or crosstalk between different parts of the system, maintaining the integrity and accuracy of the signals being transmitted. Differential isolation can be achieved through techniques such as using transformers, optocouplers, or differential signaling.


Why the water levels in the various Manometer tubes are different?

The water levels in different manometer tubes are different because they reflect the pressure differences between two points in a system. The height difference in the tubes indicates the pressure differential between the two points they are measuring. This pressure difference causes the fluid to rise or fall in each tube to balance the pressures.


What is the difference between 'collenchyma' and 'chlorenchyma'?

Difference between collenchyma and chlorenchyma