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Current FDA guidelines allow a maximum of 10.5 ml/kilogram body weight for whole blood donation in the United States. Most blood collection facilities use 500 ml whole blood collection bags, with an additional 50 ml collected for FDA mandated screening tests.

Within hours of donation, the body recruits fluid from the interstitial tissue space to replace lost volume. Red cell mass is replaced by the bone marrow within 2-4 weeks, if adequate iron stores are present.

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