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How did the appearance of the 24 hour and the 72 hour Bacillus cultures differ How do you account for this difference?
Call me delusional, but I cannot shake the feeling that there was a lot of
other stuff that was supposed to go along with this question. Like maybe
actually seeing the two cultures, knowing where the seed bacilli came from,
or knowing something about how their preparation or incubation might have
differed.
The 24-hour Bacillus culture typically appears as small colonies or growth patches on agar plates, while the 72-hour culture shows more substantial growth with larger colonies and increased density. This difference is due to the extended incubation period allowing the bacteria more time to grow and multiply, resulting in more visible and developed colonies.
A 24 hour culture of Bacillus will usually be in the logarithmic phase wherein the cells have maximum metabolic activity. On the other hand, a 72 hour culture will have passed the logarithmic phase and entered the stationary phase. Some of the cells could also possibly be in the phase of decline.
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