0
How do you convert DNA with a concentration of 660.92 ng per uL to 5000 ng per uL and end up with a total volume of 25uL?
Wiki User
∙ 14y ago
C1v1=c2v2
Wiki User
∙ 14y ago
To convert DNA with a concentration of 660.92 ng/uL to 5000 ng/uL in a total volume of 25 uL, you can calculate the volume of the original DNA required to reach the desired concentration. First, determine the amount of DNA needed in the final concentration (5000 ng/uL * 25 uL = 125,000 ng). Then, divide this amount by the original concentration to find the volume required (125,000 ng / 660.92 ng/uL ≈ 189.14 uL). Therefore, you would mix the original DNA with an appropriate volume of diluent to reach the desired concentration and then adjust the total volume to 25 uL.
Add your answer:
Earn +20 pts
What is 4 ml of chemical added to 1liter of water in many ppm concentration?
To calculate the concentration in ppm, you need to know the mass of the chemical added to the water. If you know the density of the chemical, you can convert the volume (4 mL) to mass. Then, you can calculate the concentration in ppm using the mass of the chemical and the total volume of the solution (1 liter).
How would you estimate the volume of oxygen generated in a plastic bag?
You can estimate the volume of oxygen generated in a plastic bag by knowing the concentration of the oxygen generated and the total volume of the bag. Multiply the concentration of oxygen by the total volume of the bag to get an estimate of the volume of oxygen generated.
If 0.93 mL of a 0.127 M HCl solution is diluted with water to a total volume of 10.00 mL what is the resulting concentration in M?
The resulting concentration in M is 0,0118 (approx. 0,012).
A person suffering from hyponatremia has a sodium ion concentration in the blood of 0.120 M and a total blood volume of 4.8 L?
To calculate the total amount of sodium ions in the blood, you can use the formula: total amount = concentration x volume. Therefore, in this case, the total amount of sodium ions in the blood would be 0.120 M x 4.8 L = 0.576 moles of sodium ions.
Does volume of solute effect the molarity of the solute?
No, the volume of solute does not affect the molarity of the solute. Molarity is a concentration term that is calculated based on the amount of solute (in moles) and the volume of solvent (in liters). It is independent of the total volume of the solution.
Why molarity not based on the volume of solvent alone?
Molarity is based on the total volume of the solution (solvent + solute), because the concentration of a solution is defined as the amount of solute dissolved in a given volume of the solution. By considering the total volume, we can accurately determine the concentration of the solute in the solution.
What is 4 ml of chemical added to 1liter of water in many ppm concentration?
To calculate the concentration in ppm, you need to know the mass of the chemical added to the water. If you know the density of the chemical, you can convert the volume (4 mL) to mass. Then, you can calculate the concentration in ppm using the mass of the chemical and the total volume of the solution (1 liter).
What is a concentration of a solution?
Concentration of a solution refers to the amount of solute present in a given quantity of solvent or total solution. It can be expressed in various ways, such as mass/volume (g/mL), moles/volume (mol/L), or as a percentage. Concentration is important for determining the properties and behavior of a solution.
What is the volume percent concentration of hexane in a solution made by mixing 50.0 mL of hexane with 1.0 L of pentane?
To calculate the volume percent concentration of hexane in the solution, you need to determine the total volume of the solution first. Mixing 50.0 mL of hexane with 1.0 L of pentane gives a total volume of 1.05 L (1000 mL + 50 mL). The volume percent concentration of hexane can be calculated as (volume of hexane / total volume) x 100. This gives (50 mL / 1050 mL) x 100 = 4.76% volume percent of hexane in the solution.
How would you estimate the volume of oxygen generated in a plastic bag?
You can estimate the volume of oxygen generated in a plastic bag by knowing the concentration of the oxygen generated and the total volume of the bag. Multiply the concentration of oxygen by the total volume of the bag to get an estimate of the volume of oxygen generated.
What is the volume percent concentration of hexadecimal in a solution made by mixing 50.0 ml of hexadecimal with 1.0 like of pentane?
To calculate the volume percent concentration of a component in a solution, you need to know the total volume of the solution. In this case, the total volume after mixing would be 50.0 ml + 1.0 ml = 51.0 ml. To find the volume percent of hexadecimal, you would take the volume of hexadecimal (50.0 ml) divided by the total volume of the solution (51.0 ml) and multiply by 100. This gives you a volume percent concentration of hexadecimal in the solution.
What quantities are compared when the concentration of a solution is measured?
The quantities compared when measuring the concentration of a solution are the amount of solute (substance being dissolved) and the amount of solvent (substance doing the dissolving). The concentration is typically expressed as the amount of solute per unit volume of solution, such as grams per liter.
What is the volume percent concentration of propanol in a solution made by mixing 100 mL of propanol with 0.30 L of water?
To calculate the volume percent concentration of propanol in the solution, we first need to find the total volume of the solution. This would be the sum of the volumes of propanol and water: 100 mL + 300 mL = 400 mL. The volume percent concentration of propanol in the solution is then calculated by dividing the volume of propanol by the total volume of the solution and multiplying by 100: (100 mL / 400 mL) * 100 = 25%. Therefore, the propanol concentration in the solution is 25% (v/v).
What does ppmv mean?
PPMV stands for parts per million by volume, a unit of measurement used to express the concentration of a substance in a gas. It indicates the number of parts of a particular substance per million parts of the total gas volume.
What is the percent volume of ethanol?
The percent volume of ethanol in a solution is the volume of ethanol divided by the total volume of the solution, multiplied by 100. It is commonly used to express the concentration of ethanol in alcoholic beverages or solutions.
What is the percent by volume concentration of 78.9 mL of acetone in 1550 mL of an acetone-water solution?
The total volume of the solution is 78.9 mL + 1550 mL = 1628.9 mL. To calculate the percent by volume concentration of acetone, divide the volume of acetone by the total volume of the solution and multiply by 100. In this case, (78.9 mL / 1628.9 mL) x 100 = 4.85% volume concentration of acetone.
How do you calculate chloroform concentration?
To calculate chloroform concentration, divide the mass or volume of chloroform by the total volume of the solution it is in. For example, if you have 5 grams of chloroform in 100 mL of solution, the concentration would be 5 grams / 100 mL = 0.05 g/mL or 50 mg/mL.
