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How do you separate two different proteins of same molecular weight?
Wiki User
∙ 13y ago
that two proteins having different PI value . so run that two proteins in capillary having different PI regions. then they can separate easily according to their PI value.
Wiki User
∙ 13y ago
To separate two proteins of the same molecular weight, you can use techniques such as gel electrophoresis, chromatography, or isoelectric focusing. These methods rely on differences in properties such as size, charge, or interactions with the separation matrix to effectively separate the proteins.
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Why do different proteins have the same relative molecular mass?
Different proteins can have the same relative molecular mass because molecular weight alone does not determine a protein's structure or function. Proteins can vary in amino acid sequence, post-translational modifications, and three-dimensional structure while still having the same molecular mass. Thus, proteins with different compositions and functions can have similar molecular masses.
What is sds-page used for?
SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) is a common technique used to separate proteins based on their molecular weight. It denatures the proteins and binds a negative charge to them, allowing for separation solely based on size. It is often used in biochemistry and molecular biology research to analyze protein composition and purity.
How do you calculate molecular weight in electrophoresis?
To calculate the molecular weight of a protein in electrophoresis, you would use a standard curve generated with protein standards of known molecular weights run on the same gel. By plotting the migration distance of the standard proteins against their known molecular weights, you can then determine the molecular weight of your protein of interest based on its migration distance on the gel in comparison to the standard curve.
What is the molecular weight of Bacillus Megaterium?
The molecular weight of Bacillus megaterium will vary depending on the specific proteins, DNA, RNA, and other molecules present in its cellular structure. Generally, the molecular weight of Bacillus megaterium is estimated to be around 5-10 million Daltons.
What solute has the highest molecular rate?
The compound with the highest molar mass is likely to have the highest molecular weight as well. Examples of solutes with high molecular weights include proteins like albumin or polysaccharides like starch.
Why do different proteins have the same relative molecular mass?
Different proteins can have the same relative molecular mass because molecular weight alone does not determine a protein's structure or function. Proteins can vary in amino acid sequence, post-translational modifications, and three-dimensional structure while still having the same molecular mass. Thus, proteins with different compositions and functions can have similar molecular masses.
What has higher molecular weight amino acid or protein?
Proteins have the higher molecular weight. They consist of long chains of amino acids joined together.
What does an IPG strip do?
it separates proteins by their isoelectric point along an pH gradient imobilized by a polyacrilamide gel. it is usually the first dimension of separation before using an SDS-PAGE technique to then separate proteins based on molecular weight (2nd dimension).
How separated bands of human serum in a sds page can be recognised?
it could be recognized using a staining day that used to stain proteins in the sample it will separate several band according to molecular weight
What is sds-page used for?
SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) is a common technique used to separate proteins based on their molecular weight. It denatures the proteins and binds a negative charge to them, allowing for separation solely based on size. It is often used in biochemistry and molecular biology research to analyze protein composition and purity.
What is protein gel..?
A protein gel is a matrix used in gel electrophoresis to separate proteins based on their size and charge. It is made of a cross-linked polymer that forms a porous network through which proteins can migrate when an electric field is applied. The proteins move through the gel at different rates, allowing for their separation and analysis.
What is a molecule of low molecular weight capable of reacting with identical or different molecules of low molecular weight to form a polymer?
This is a catalyst.
What is the molecular weight of Bacillus Megaterium?
The molecular weight of Bacillus megaterium will vary depending on the specific proteins, DNA, RNA, and other molecules present in its cellular structure. Generally, the molecular weight of Bacillus megaterium is estimated to be around 5-10 million Daltons.
What can you infer about large molecular-weight proteins that cannot be transported into the nucleus?
Large molecular-weight proteins that cannot be transported into the nucleus likely do not contain a nuclear localization signal (NLS) that is recognized by importins. These proteins may function primarily in the cytoplasm or at the cell membrane, where they carry out their specific roles without the need to access the nucleus.
What is the molecular weight of butter?
The molecular weight of butter is approximately 800-1000 g/mol. Butter is a mixture of various fats, with the main components being triglycerides made up of fatty acids, which contribute to its overall molecular weight.
Does a protein solution have a high molarity?
No. The reason is that low molecular weight compounds tend to have high molarity. As an example NaF is 42.5 molecular weight. So 42.5g dissolved in 1 liter of water would only be 4.25% but be 1 molar. Proteins tend to have every high molecular weight. So if a protein was say 1000 molecular weight, a 10% solution would contain 100g but only be 0.1 molar.
Why native gel electrophoresis can be used for determining the molecular weight of proteins?
No! native gels are used to run the proteins in native form, this will tell about the protein's mulimeric nature (ie.monomer or dimer or tetramer etc..).
