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If you want to read the absorbance at 595nm of a protein solution mixed with the colorizing solution called Bradford reagent what is a suitable reagent blank?
Wiki User
∙ 16y ago
a "blank" cuvette can be made with a sufficient amount of distilled water (assuming you're diluting your stock solution with distilled water).
Wiki User
∙ 16y ago
A suitable reagent blank for measuring the absorbance of a protein solution mixed with Bradford reagent at 595nm would be a blank containing all components of the reaction except the protein sample, such as water or buffer mixed with the Bradford reagent. This blank will account for any background absorbance contributed by the reagent itself, allowing for a more accurate measurement of the protein concentration.
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