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Is coryne bacterium is oxidase positive?
No, Corynebacterium species are typically oxidase-negative. This means they do not produce the enzyme cytochrome c oxidase, which is essential for the oxidase test.
Why is an oxidase test performed on a culture before using API 20E and Enterotube II to identify the culture?
The oxidase test is performed to determine if the culture contains cytochrome c oxidase enzyme, which helps in distinguishing between oxidase-positive and oxidase-negative organisms. This information is important for selecting the appropriate biochemical tests in the API 20E and Enterotube II identification systems, as these tests are designed to work best with specific types of bacteria based on their oxidative characteristics.
What is the Lactococcus lactis ssp lactis oxidase test result?
The oxidase test result for Lactococcus lactis ssp lactis is negative. This bacterium lacks the enzyme cytochrome c oxidase that is needed to produce a positive result in the oxidase test.
Can an aerobic organsm be oxidase negative?
Yes, some aerobic organisms can be oxidase negative. Oxidase positivity is not always directly related to aerobicity, as it depends on the presence of cytochrome c oxidase in the organism. Some aerobic bacteria lack this enzyme and are therefore oxidase negative.
Why is it advisable to include a known oxidase-positive control in any test of an unknown organism?
Including an oxidase-positive control in a test of an unknown organism helps to confirm the presence of the enzyme oxidase in the test system. This control provides a baseline for comparison with the unknown organism to determine if it also produces oxidase. This is particularly important in biochemical testing to accurately identify the unknown organism based on its metabolic properties.
