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Absorbance measurements are taken at λ max because it represents the wavelength at which a compound absorbs light most strongly, allowing for more accurate and sensitive detection of the compound. Additionally, λ max provides specific information about the electronic structure of the compound, aiding in its identification and characterization.
Absorbance at 750 nm in Lowry's method is used because it corresponds to the peak absorbance of the copper-tyrosine complex formed during the reaction, ensuring accurate measurement of the protein concentration. This wavelength specifically targets the color change associated with the biuret reaction, enhancing the sensitivity and specificity of the assay.
Some common materials used in measurements include rulers, tape measures, calipers, micrometers, scales, thermometers, pH meters, and spectrophotometers. These materials are selected based on the type of measurement being taken and the level of precision required.
During a cardiac catheterization, blood measurements taken from the left ventricle may include oxygen content, pressure, and the presence of certain markers related to heart function or damage, such as troponin levels. These measurements help in assessing the heart's pumping capacity and overall function.
Probable you think to the evaporation during measurements.
A datum point is a fixed reference point used in various activities such as surveying, drafting, and engineering to establish a baseline or starting point for measurements and calculations. It helps ensure consistent and accurate results by providing a known reference for all measurements taken within a specific project or task.