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Why absorbance should be taken in 750 nm in lowrys method?

Absorbance at 750 nm in Lowry's method is used because it corresponds to the peak absorbance of the copper-tyrosine complex formed during the reaction, ensuring accurate measurement of the protein concentration. This wavelength specifically targets the color change associated with the biuret reaction, enhancing the sensitivity and specificity of the assay.


What are the different materials used in measurements?

Some common materials used in measurements include rulers, tape measures, calipers, micrometers, scales, thermometers, pH meters, and spectrophotometers. These materials are selected based on the type of measurement being taken and the level of precision required.


What blood measurements are taken of the left ventricle during a cardiac catheterization?

During a cardiac catheterization, blood measurements taken from the left ventricle may include oxygen content, pressure, and the presence of certain markers related to heart function or damage, such as troponin levels. These measurements help in assessing the heart's pumping capacity and overall function.


If the volume of the water could be measured very accurately there would still be uncertainty in the mass of the wate. What source of error have we not taken in account?

Probable you think to the evaporation during measurements.


How is a datum point used?

A datum point is a fixed reference point used in various activities such as surveying, drafting, and engineering to establish a baseline or starting point for measurements and calculations. It helps ensure consistent and accurate results by providing a known reference for all measurements taken within a specific project or task.

Related Questions

Why are some of the reasons for discrepancies between your classmates measurements of identical features on the megabloks?

Some possible reasons for discrepancies in measurements could be human error in recording or taking the measurements, variations in technique or tools used, differences in interpretation of where the measurement should be taken, or discrepancies in calibration of measuring instruments.


What is the level of uncertainty associated with measurements taken using a digital scale?

The level of uncertainty associated with measurements taken using a digital scale is generally low, as digital scales provide precise and accurate measurements.


Why absorbance should be taken in 750 nm in lowrys method?

Absorbance at 750 nm in Lowry's method is used because it corresponds to the peak absorbance of the copper-tyrosine complex formed during the reaction, ensuring accurate measurement of the protein concentration. This wavelength specifically targets the color change associated with the biuret reaction, enhancing the sensitivity and specificity of the assay.


What two measurements are taken during and after an earthquake?

An foreshocks and aftershocks


What were the officials who measured the depth of the River Nile?

In its very long history and given it flows over 4,000 miles through different countries, there must have been thousands of measurements taken for hundreds of reasons.


What is a fixed reference point?

A set point where all measurements can be taken from


How warm is a tornado on the inside?

That is not known. Not enough measurements have been taken.


What is statistical unit?

It is the unit - person, household, object - for which measurements are taken.


What is the difference between direct and indirect measurement?

Direct is an exact measurement and indirect is an estimate.


Measurements taken from space show the earth to be?

Measurements taken from space show the Earth to be round in shape, with a diameter of approximately 12,742 kilometers at the equator. The Earth's circumference is approximately 40,075 kilometers.


What measurements are typically taken during a hair cut to ensure precision and accuracy?

During a haircut, measurements such as length, angle, and balance are typically taken to ensure precision and accuracy. These measurements help the stylist create a balanced and well-proportioned hairstyle that meets the client's desired look.


Two measurements that could be taken to determine a organism's size?

length and weight