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Why absorbance should be taken in 750 nm in lowrys method?

Absorbance at 750 nm in Lowry's method is used because it corresponds to the peak absorbance of the copper-tyrosine complex formed during the reaction, ensuring accurate measurement of the protein concentration. This wavelength specifically targets the color change associated with the biuret reaction, enhancing the sensitivity and specificity of the assay.


What is the term for the measurements taken by a scientist?

The term for the measurements taken by a scientist is "data." Data can be quantitative, involving numerical values, or qualitative, involving descriptive characteristics. It serves as the foundation for analysis and interpretation in scientific research.


What are the precautions to be taken in the beer Lambert experiment?

In the Beer-Lambert experiment, it is essential to ensure that the cuvettes used are clean and free of scratches to avoid scattering of light. The light source should be stable and properly aligned with the detector to ensure accurate measurements. Additionally, the solution concentration must be within the linear range of the Beer-Lambert law, and the path length of the cuvette must be consistent throughout the experiment. Lastly, all measurements should be conducted at a constant temperature to prevent variations in absorbance.


What are the different materials used in measurements?

Some common materials used in measurements include rulers, tape measures, calipers, micrometers, scales, thermometers, pH meters, and spectrophotometers. These materials are selected based on the type of measurement being taken and the level of precision required.


What blood measurements are taken of the left ventricle during a cardiac catheterization?

A comparison of how much blood is ejected from the heart's left ventricle during its contraction phase with a measurement of blood remaining at the end of the left ventricle's relaxation phase.

Related Questions

Why are some of the reasons for discrepancies between your classmates measurements of identical features on the megabloks?

Some possible reasons for discrepancies in measurements could be human error in recording or taking the measurements, variations in technique or tools used, differences in interpretation of where the measurement should be taken, or discrepancies in calibration of measuring instruments.


When are measurements taken?

Measurements are typically taken at specific points in time or under certain conditions, depending on the purpose of the measurement. For scientific experiments, measurements are often recorded at regular intervals or during key phases of the experiment. In everyday contexts, such as cooking or construction, measurements are taken before starting a task to ensure accuracy. Additionally, measurements can also be taken for monitoring changes over time, like in health assessments or environmental studies.


Why absorbance should be taken in 750 nm in lowrys method?

Absorbance at 750 nm in Lowry's method is used because it corresponds to the peak absorbance of the copper-tyrosine complex formed during the reaction, ensuring accurate measurement of the protein concentration. This wavelength specifically targets the color change associated with the biuret reaction, enhancing the sensitivity and specificity of the assay.


What is the level of uncertainty associated with measurements taken using a digital scale?

The level of uncertainty associated with measurements taken using a digital scale is generally low, as digital scales provide precise and accurate measurements.


What were the officials who measured the depth of the River Nile?

In its very long history and given it flows over 4,000 miles through different countries, there must have been thousands of measurements taken for hundreds of reasons.


What two measurements are taken during and after an earthquake?

An foreshocks and aftershocks


What is a fixed reference point?

A set point where all measurements can be taken from


How warm is a tornado on the inside?

That is not known. Not enough measurements have been taken.


What is statistical unit?

It is the unit - person, household, object - for which measurements are taken.


What is the difference between direct and indirect measurement?

Direct is an exact measurement and indirect is an estimate.


Measurements taken from space show the earth to be?

Measurements taken from space show the Earth to be round in shape, with a diameter of approximately 12,742 kilometers at the equator. The Earth's circumference is approximately 40,075 kilometers.


What is the term for the measurements taken by a scientist?

The term for the measurements taken by a scientist is "data." Data can be quantitative, involving numerical values, or qualitative, involving descriptive characteristics. It serves as the foundation for analysis and interpretation in scientific research.