0
Add your answer:
Earn +20 pts
Why volatile compounds are analysed in gc but not in hplc?
GC can give very resolved sharp peaks with short run time compared to hplc. additionally, there is less compatibility issue in setting an MS up to a GC than HPLC
What type of species can be separated by HPLC but not by gas liquid chromatography?
mixture of enantiomers can be separated by HPLC
What is the storage area for urine?
Bladder
Can melamine detect by HPLC?
Yes, melamine can be detected by HPLC (High Performance Liquid Chromatography). HPLC is a common analytical technique used to separate and quantify compounds in a mixture, including melamine. Detection methods such as UV-Vis spectroscopy or mass spectrometry can be used in conjunction with HPLC to identify and quantify melamine accurately.
Is there any way to get samples of dark matter?
NO we can not get the samples of dark matter as it is the type of matter in astronomy and cosmology that can not be even seen with telescopes.
Why volatile compounds are analysed in gc but not in hplc?
GC can give very resolved sharp peaks with short run time compared to hplc. additionally, there is less compatibility issue in setting an MS up to a GC than HPLC
How do you know if pollution has affected a place?
you first have to define what type of pollution. Common ones are air, soil and water. GCMS can be used on air sample tubes or sample trains for flue gas. Soil samples are best analysed by ICP-MS for elemental quantitation. Water samples are often analysed by LC-MS, sometimes by GC-MS
What type of species can be separated by HPLC but not by gas liquid chromatography?
mixture of enantiomers can be separated by HPLC
Where the urine stored?
In the bladder. Or if that's the question: Urine samples should be stored in the fridge if they can't get analysed immediatly.
Definition of standard in HPLC?
In HPLC, a standard is a known compound with a defined chemical structure and purity used for comparison and identification purposes. Standards are essential for calibrating instruments, determining retention times, and quantifying unknown compounds in samples during analysis.
What is an HPLC column?
HPLC Column is one type of tube containing a stationary phase react with mobile phase to detect peak
How would forensic scientist detect codeine?
HPLC (high pressure liquid chromatography), hair samples and bodily fluids can be tested this way.
Does HPLC can read histamine and TVB-N?
Yes, HPLC can be used to analyze histamine and TVB-N (Total Volatile Basic Nitrogen) in food samples. HPLC is a sensitive and selective technique that can separate and quantify various compounds, including histamine and TVB-N, based on their chemical properties. Pre-column derivatization may be required for some compounds to enhance their detection sensitivity in HPLC analysis.
What investigations are conducted in forensic labs?
Forensic labs have many purposes, the general use is to analyse gathered evidence and clarify facts. biological samples can be analysed to get a "DNA fingerprint". mobile phones and electrical equipment such as computers are also analysed. there are many more purposes these are just a few
How do you calibrate for HPLC?
To calibrate an HPLC system, you typically use a calibration standard containing known concentrations of target compounds. Inject the standard into the HPLC system, establish calibration curves by plotting peak area vs concentration, and use this to quantify unknown samples. Regular calibration is important for ensuring accuracy and precision in HPLC analysis.
How do you distinguised np-hplc and rp-hplc?
NP-HPLC (normal phase HPLC) separates compounds based on their polarity, where the stationary phase is polar and the mobile phase is nonpolar. RP-HPLC (reverse phase HPLC) separates compounds based on their hydrophobicity, where the stationary phase is nonpolar and the mobile phase is polar. RP-HPLC is more commonly used due to its versatility and ability to handle a wider range of compounds.
What are the key differences between FPLC and HPLC, and how do these differences impact their respective applications in chromatography?
FPLC (fast protein liquid chromatography) and HPLC (high-performance liquid chromatography) are both chromatography techniques used to separate and analyze compounds. The key differences lie in their intended applications and the types of samples they are best suited for. FPLC is primarily used for purifying proteins and other biomolecules, while HPLC is more versatile and can be used for a wider range of compounds including small molecules, peptides, and proteins. FPLC typically operates at lower pressures and flow rates compared to HPLC, making it gentler on biomolecules. These differences impact their applications in chromatography by determining which type of sample each technique is best suited for. FPLC is ideal for purifying proteins and biomolecules, while HPLC is better suited for a broader range of compounds. The choice between FPLC and HPLC depends on the specific requirements of the experiment and the nature of the sample being analyzed.
