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All agar plates are incubated at all temps because that is the best temperature for those particular bacteria to grow and reproduce quickly so a person can see results. Some agar plates get incubated at room temp around 22-24c so only certain bacteria will grow, or 30c so only yeast will grow or 37c so only E. coli will, the temp depends on what bacteria is being grown.
Blood agar plates are incubated at 35-37 degrees Celsius because this temperature mimics the human body temperature, providing a suitable environment for the growth of pathogens. Incubating at this temperature also promotes the optimal growth of many pathogenic bacteria, allowing for better detection and isolation of microbial organisms in the sample.
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What does -35 micrograms of alcohol in 100milliliters mean?
This means there are 35 micrograms of alcohol per 100 milliliters of blood. It's typically used as a measure of blood alcohol concentration (BAC) to determine levels of intoxication. A BAC of -35 micrograms would indicate a negative alcohol level, which is not physiologically possible.
What is the shelf life of whole collected blood when stored at at 4 degrees C A 206 days B120 days C 55 days D 35 days?
A 35 days
What percentage of red blood cells in a sample of human blood is normally about?
5.72 million red blood cells per microliter (mcL) of blood for men and 5.03 million per mcL for women. In healthy men, 41 percent to 50 percent of blood is red blood cells. In healthy women, 35 percent to 46 percent is red blood cells
What is the composition of starch agar?
Composition * Dehydrated powder, hygroscopic in nature, store in a dry place, in tightly-sealed containers below 25°C and protect from direct Sunlight. Instructions for Use Dissolve 45gms in 445 ml distilled water. Gently heat to boiling with gentle swirling and dissolve the medium completely. Sterilize by autoclaving at 15 psi (121°C) for 15 minutes. Cool to 45-50°C. Aseptically add equal amount (445ml) of sterile 2% Haemoglobin powder (TS 021) and add 10ml of Vitamins growth supplement (TS 022) or Yeast autolysate supplement (TS 023). Mix well. Dispense into sterile Petri plates or sterile culture tubes. Appearance: Light amber color, clear to slightly opalescent gel. On addition of Haemoglobin: Chocolate brown colour appears, opaque gel pH (at 25°C): 7.3 ± 0.2 Principle CHOCHOLATE AGAR BASE is used for isolation and cultivation of fastidious microorganisms like Neisseria gonorrhoeae. It can also be used for cultivation of aerobic, anaerobic and microaerophillic microorganisms. Medium contains Proteose peptone is a nitrogen source required for the growth of wide variety of organisms. Dextrose acts a carbon energy source. Disodium phosphate buffers the medium whereas sodium chloride maintains the osmotic equilibrium. Agar is the solidifying agent. This medium is supplemented with cofactor, which provides NAD to facilitate the growth of Haemophilus influenzae, Neisseria gonorrhoeae and Neisseria meningitidis. Heated sheep blood is added to give the medium its "chocolate" appearance. This medium is prepared, stored and dispensed under oxygen-free conditions to prevent the formation of oxidized products prior to use. Interpretation Cultural characteristics observed after inoculating (103CFU/ml), on incubation at 35 ± 2°C for 48 hours with CO2. Ingredients Gms/Ltr. Proteose peptone 20.0 Agar 15.0 Sodium chloride 5.00 Disodium phosphate 5.00 Dextrose 0.50 Regards NITIN MALHOTRA
What percentage of the population shares type A positive blood?
Approximately 35-40% of the population has type A positive blood.
How will you check the sterility of the media?
Agar is a medium, so you are checking the sterility of the agar. After preparation one usually places an agar plate at room temperature and another agar plate at 35 to 37 degrees C. After 24-48 hrs of incubation a visual check is made to see if there is any visible growth on the uninoculated plates. If you are adding blood, etc. to the agar, those components can be checked by subbing them to a blood agar plate to see if there is any growth--which would indicate non-sterile components.
How are Petri dishes sterilized?
Petri dishes are typically sterilized by being exposed to high heat in an autoclave or by using chemical agents such as ethylene oxide gas. This process helps to eliminate any microorganisms present on the surface of the dish, ensuring that the culture medium remains uncontaminated during experimentation.
How much is two 45 pound plates and two 35 pound plates on an olympic bar weigh?
205
What does a distolic pressure of 35 mean?
The pressure of blood returning to the heart; 35 is low.
What is the value of a spode victorian christmas plate No 3 sleighing?
Plates for sleighing are roughly $30 - $35.
How many different license plates exist if each license plate contains 3 letters and 4 numbers and all four numbers are even?
384,475,000 license plates. There are 35 different letter/number combinations possible. Each combination has 10,985,000 variants. 35*10,985,000 = 384,475,000
Can Surgical anesthesia is equal to what blood alcohol level?
.35
What are the release dates for 1st Look - 2008 Young Blood 1-35?
1st Look - 2008 Young Blood 1-35 was released on: USA: 8 August 2009
What is cos (35)?
Normal CO2 in the blood is the same as Normal PH. Ph is 7.35-7.45 CO2 is 35-45
What is the ideal blood pressure for a 35 year old?
110/80
What does a mixed venous blood gas sample drawn from pulmonary artery of 35 indicate?
It depends if the 35 is oxygen 35 mmHg or 35% oxygen saturation. Most likely you mean 35 mmgHg, correlating to a oxygen saturation about 65%, which is around normal. This blood has the lowest oxygen content in the body system. A 35% saturation though is clearly abnormal most likely indicates the body is not getting adequate oxygen.
What are the release dates for Mr- and Mrs- North - 1952 Salt in His Blood 1-35?
Mr- and Mrs- North - 1952 Salt in His Blood 1-35 was released on: USA: 29 May 1953
