All agar plates are incubated at all temps because that is the best temperature for those particular bacteria to grow and reproduce quickly so a person can see results. Some agar plates get incubated at room temp around 22-24c so only certain bacteria will grow, or 30c so only yeast will grow or 37c so only E. coli will, the temp depends on what bacteria is being grown.
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Blood agar plates are incubated at 35-37 degrees Celsius because this temperature mimics the human body temperature, providing a suitable environment for the growth of pathogens. Incubating at this temperature also promotes the optimal growth of many pathogenic bacteria, allowing for better detection and isolation of microbial organisms in the sample.
This means there are 35 micrograms of alcohol per 100 milliliters of blood. It's typically used as a measure of blood alcohol concentration (BAC) to determine levels of intoxication. A BAC of -35 micrograms would indicate a negative alcohol level, which is not physiologically possible.
A 35 days
5.72 million red blood cells per microliter (mcL) of blood for men and 5.03 million per mcL for women. In healthy men, 41 percent to 50 percent of blood is red blood cells. In healthy women, 35 percent to 46 percent is red blood cells
Composition * Dehydrated powder, hygroscopic in nature, store in a dry place, in tightly-sealed containers below 25°C and protect from direct Sunlight. Instructions for Use Dissolve 45gms in 445 ml distilled water. Gently heat to boiling with gentle swirling and dissolve the medium completely. Sterilize by autoclaving at 15 psi (121°C) for 15 minutes. Cool to 45-50°C. Aseptically add equal amount (445ml) of sterile 2% Haemoglobin powder (TS 021) and add 10ml of Vitamins growth supplement (TS 022) or Yeast autolysate supplement (TS 023). Mix well. Dispense into sterile Petri plates or sterile culture tubes. Appearance: Light amber color, clear to slightly opalescent gel. On addition of Haemoglobin: Chocolate brown colour appears, opaque gel pH (at 25°C): 7.3 ± 0.2 Principle CHOCHOLATE AGAR BASE is used for isolation and cultivation of fastidious microorganisms like Neisseria gonorrhoeae. It can also be used for cultivation of aerobic, anaerobic and microaerophillic microorganisms. Medium contains Proteose peptone is a nitrogen source required for the growth of wide variety of organisms. Dextrose acts a carbon energy source. Disodium phosphate buffers the medium whereas sodium chloride maintains the osmotic equilibrium. Agar is the solidifying agent. This medium is supplemented with cofactor, which provides NAD to facilitate the growth of Haemophilus influenzae, Neisseria gonorrhoeae and Neisseria meningitidis. Heated sheep blood is added to give the medium its "chocolate" appearance. This medium is prepared, stored and dispensed under oxygen-free conditions to prevent the formation of oxidized products prior to use. Interpretation Cultural characteristics observed after inoculating (103CFU/ml), on incubation at 35 ± 2°C for 48 hours with CO2. Ingredients Gms/Ltr. Proteose peptone 20.0 Agar 15.0 Sodium chloride 5.00 Disodium phosphate 5.00 Dextrose 0.50 Regards NITIN MALHOTRA
Approximately 35-40% of the population has type A positive blood.