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Well UC Student, counting a plate between 20 and 200 colonies provides an accurate estimation of the total number of bacteria. Counting plates with less than 20 colonies would providen unreliable data, while plates with 200 or more colonies is too difficult to do with the human eye.

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Plates with 25 to 250 colonies are used for calculations to ensure accuracy and reliability of the results. Having a colony count within this range helps to minimize error and provides a statistically significant sample size for analysis. Plates with colony counts outside of this range can make it difficult to accurately determine the concentration of microorganisms in a sample.

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10mo ago
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A plate with less than 25 colonies is inaccurate because a single contaminant could influence the results.

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12y ago
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Q: Why are plates with 25 to 250 colonies used for calculations?
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