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What is front end estimation?
Front end estimation- An estimation method in which the front digits are added or subtracted
What is the method of making a rough measurement?
A method of making a rough measurement is: Estimation Answered by :PopTy13
How do you compute a square root?
Square roots are computed using the Babylonian method, calculators, Newton's method, or the Rough estimation method. * * * * * Or the Newton-Raphson method.
Role of F C reagent in protein estimation?
phosphomolybdic & phosphotungstic components in FC reagents gets reduced by certain amino acids present in the protein like tyrosine , tryptophan thereby resulting in the production of a a blue colored compound . Estimation of this compound in turn provides the amount of protein present in the sample.
What is the Front-end estimation method?
Front end estimation is a quick method to find an estimate of sums and differences, however it is generally not more accurate than the answer produced by adding or subtracting rounded numbers. Take for example a pair of pants and a shirt that are $24.99 and $16.99, respectively. The correct total of these items would be $41.98. Using the method of adding rounded numbers would yield $42 - which is only two cents from the correct total. Front end estimation would yield $40 - which is $1.98 short of the correct total. The use of front end estimation is why stores sell things for $24.99 and $16.99, rather than $25 and $17. It is an open attempt to make the consumer think that they are paying for something at a lower price than they actually are.
Is lowry's protein estimation an invasive method?
No, Lowry's protein estimation method is not invasive. It is a biochemical method used to quantify the total protein concentration in a sample based on the reaction of proteins with specific reagents. It does not involve physical penetration or manipulation of the sample.
How long the blue colour is stable in protein estimation by lowry's method?
60 minutes 60 minutes
What method are used for the estimation of molecular weight of protein?
ofcoz the protein migrate so long as it is charged ,once it become neutral it will stop migrating
Is lowry's method an invasive method?
No. It is a non-invasive method
What is front end estimation?
Front end estimation- An estimation method in which the front digits are added or subtracted
What is protein estimation?
here the amount of protein in a sample can be determined, using different protocols, wherein the reagent mixture( ex: FC reagent) which when added to the sample containing protein reacts with the specific amino acids giving colour, thus the amount of protein in a sample can be estimated and the data used for further protein studies.
What is a method for math in a estimation?
round or rounding
Why BSA is used as standard for protein estimation?
The Bradford assay (BSA) is commonly used for protein estimation because it is quick, sensitive, and compatible with a wide range of proteins. BSA is a reliable method for measuring protein concentration due to its colorimetric detection of the dye-protein complex, making it a popular choice in biochemical research and diagnostics.
Why protein estimation is done at 660 nanometer in folin lowry method?
Protein estimation in the Folin-Lowry method is done at 660 nanometers because this wavelength corresponds to the highest absorption peak of the complex formed between proteins and the Folin-Lowry reagent. This wavelength is ideal for accurately measuring the concentration of proteins present in a sample based on the colorimetric reaction that occurs.
Why we are used sodium bicarbonate for the estimation of protein?
Sodium bicarbonate is not necessarily used in the estimation of protein. There are very many protein assays on the market, and the vast majority of them do NOT use NaHCO3. Look up Lowry, Biuret, Bradford, etc.
Different methods of protein estimation?
This question has to be answered depending upon the meaning of a research project that involves proteins. The estimation of proteins can be done to know the protein fraction of a sample collected from the field, either if the protein content will be isolated to be studied, or to remove protein fraction from the sample (sometimes the investigator does not want that proteolytic enzymes chew a target molecules or cellular structures). Another application is when an enzymatic reaction is going to be performed to digest a particular protein in an aliquot, the protein content is crucial for stoichometric purposes. On the other hand, if the research imply the work with nucleic acids, is very important "to inactivate" the nucleolytic enzymes present in cytoplasmic fluids from lysed cells with specific inactivators, chemical or biochemical, added in correct levels according to protein contents.
What is lambda max for colorless proteins?
if the protein is colorless then it is quite tough to calculate the lambda max or absorption maxima directly (still you can check it by absorbtion maxima calculation/experiment). Otherwise, for its estimation first you have to treat it with any reagent (like folin reagent) then it will form a colored complex and then you can calculate it. For the common estimation of protein, there is a "Lowry Method" where you can calculate it on 750 wavelength.
