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What is the advantage of allowing the eluent front to rise near the top of the TLC sheet rather than stopping when only half way up?
Wiki User
∙ 13y ago
When you calculate RF values, you need the distance moved by the dye (or whatever you're using) and the distance moved by the solvent (the eluent front)
Given that no matter where the eluent front stops your measurement will always have the same standard error (say +- 1mm if you're using TLC plates and a normal ruler), then the further your eluent front and dye move, the less that measurement error will impact on your RF value - the error will be a smaller % of the overall distance.
Wiki User
∙ 13y ago
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