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75% of Jigglypuffs are females, 25% of them are males.
In Pokemon Stadium 1 and 2 Jigglypuff is a female.
In Super Smash Bros Jigglypuff is probably a female because Jigglypuff has a bow in diffrent colors.
In Super Smash Bros Melee Jigglypuff is probably a female because Jigglypuff has a flower, bow and Prinsess Peach crown in diffrent colors.
In Super Smash Bros Brawl Jigglypuff is probably a female because Jigglypuff has a female-hat in diffrent colors.
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