Convert the velocity Vmax from ΔA/min to nmol/min (milliunits, mU).
For example:
1 Unit = 1 U = 1 μmol PNP formed per min
1 milli-Unit = 1 mU = 1 nmol PNP formed per min
Extinction coefficient ε for PNP at 410 nm = 15,000 M-1 cm-1
And the pathlength = 0.34 cm
if the velocity v is determined to be 0.294 ∆A/min (absorbance units per min), then:
0.294 ∆A /min * M/(15,000 A * 0.34 cm) = 0.0000196 M/min = 19.6 μM/min = = 19.6 μmol / (liter * min) = 0.0196 μmol / (ml * min)
The enzymatic reaction was carried out in 250 μl solution, hence the velocity of the enzyme is
0.0196 μmol / (ml * min) * 0.25 ml = 0.0049 μmol/min = 4.9 nmol/min PNP generated. v = 4.9 nmol/min = 4.9 mU
If the velocity is constant, thenDisplacement = (initial velocity) multiplied by (time)
This is difficult. We are not told what it is we are to calculate. We are not told how the velocity is changing (which it does, implied by the word "initial"). Suggest re-writing the question.
Acceleration = Final velocity - Initial velocity / time
v2 - u2 = 2as so that a = (v2 - u2)/2s where u = initial velocity v = final velocity s = distance a = acceleration
The initial acceleration of an object can be found by calculating the change in velocity over time. This can be done by dividing the final velocity by the time taken to reach that velocity. The formula for initial acceleration is: initial acceleration = (final velocity - initial velocity) / time.
If the velocity is constant, thenDisplacement = (initial velocity) multiplied by (time)
Use s=ut+0.5at^2 (^2 notation for squared)Or calculate the final velocity from the known variables (Initial Velocity, Acceleration and Time)v=u+at Where V = Final Velocity, u = Initial Velocity, a = Acceleration, t = TimeThen calculate displacement (s) using s=0.5(u+v)t
This is difficult. We are not told what it is we are to calculate. We are not told how the velocity is changing (which it does, implied by the word "initial"). Suggest re-writing the question.
No. That's only one of several possibilities. -- with initial velocity, distance, and time, you can calculate acceleration -- with final velocity, distance, and time, you can calculate acceleration -- with force and mass, you can calculate acceleration -- with initial and final momentum, you can calculate acceleration -- with initial and final kinetic energy, you can calculate acceleration -- with mass, velocity at either end, and kinetic energy at the other end, you can calculate acceleration And I'm sure there are several more that I've missed.
Acceleration is an object's change in velocity divided by its change in time. So: acceleration=(final velocity - initial velocity)/(final time - initial time)
There are different formulae for calculating these variables which depend on what information is available.
change in time, initial velocity and final velocity
There is not enough information to calculate the answer.
Accelaration= change in velocity/time taken OR Acceleration=final velocity- initial velocity/time taken
?Initial velocity?
Acceleration = Final velocity - Initial velocity / time
If s = displacement, u = initial velocity, a = acceleration, t = time. Then s = ut + 1/2at2 Be careful to keep units consistent