Standard curves are used to determine the concentration of substances. First you perform an assay with various known concentrations of a substance you are trying to measure. The response might be optical density, luminescence, fluorescence, radioactivity or something else. Graph these data to make a standard curve - concentration on the X axis, and assay measurement on the Y axis.
Also perform the same assay with your unknown samples. You want to know the concentration of the substance in each of these unknown samples.
To analyze the data, fit a line or curve through the standards. For each unknown, read across the graph from the spot on the Y-axis that corresponds to the assay measurement of the unknown until you intersect the standard curve. Read down the graph until you intersect the X-axis. The concentration of substance in the unknown sample is the value on the X-axis.
In the example below, the unknown sample had 1208 counts per minute, so the concentration of the hormone is 0.236 micromolar.
Prism makes it very easy to fit your standard curve, and to read (interpolate) the concentration of unknown samples.
depends what graph....
i need a graph of some sort for a essay
The graph moves to the left.
The slope of the graph line or curve.
it is a line on a graph that is curved
To prepare the standard curve you will need linear graph paper, semi-log graph paper and absorbance. You can define your standard curve by finding the absorption or percent plot on the Y axis.
A Cooling curve graph changes shape.
The bell curve graph is another name for a normal (Gaussian) distribution graph. A Gaussian function is a certain kind of function whose graph results in a bell-shaped curve.
depends what graph....
Biologists assume that the systems they are studying are "representative" of the population as a whole, that is, that the population's test results will be distributed according to the standard curve...ie, that graph of scores that best covers the range of 90% of scores in the entire population. In short, the standard curve provides the baseline by which to compare test results.
Indifferent curve is a graph. a graph that shows the difference on the sales of two products or commodities...
The graph if a function can be a curve, but it can also be any one of a ton of other shapes.
Indifferent curve is a graph. a graph that shows the difference on the sales of two products or commodities...
The momentum-time graph is the integral of the force-time graph. that is, it is the area under the curve of the f-t graph.The momentum-time graph is the integral of the force-time graph. that is, it is the area under the curve of the f-t graph.The momentum-time graph is the integral of the force-time graph. that is, it is the area under the curve of the f-t graph.The momentum-time graph is the integral of the force-time graph. that is, it is the area under the curve of the f-t graph.
The shaped graph that is associated with polygenic inheritance is a bell curve graph. The majority of these graphs are also combined with a bar graph under the curve.
yes
i need a graph of some sort for a essay