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If you have 0.05 ml of an undiluted culture at a concentration of 3.6x10 6 CFU ml You then add 4.95 ml sterile diluent What is the dilution factor and what is the final concentration of cells?
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∙ 13y ago
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∙ 13y ago
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How do you do a serial dilution of a specific concentration of culture?
bacterial cell numbers needs reducing ,which is done by repeatedly diluting the amount of you have in your sample. A small amount of bacterial sample is mixed with a diluent solution(such as sterile broth), and then dilution are made. by adding small amount of diluted bacteria samples then spread onto the agar plate by L-shaped glass rod.
What is Trituration?
trituration:when a measurable quantity of active ingredient is diluted with inert diluent
How do you dilute trypsin10x?
To dilute trypsin 10x, you can combine 1 part of the 10x concentrated trypsin solution with 9 parts of a suitable diluent, such as PBS or cell culture media. For example, add 1 mL of the 10x trypsin to 9 mL of the diluent to obtain a 1x trypsin solution. Mix thoroughly before use.
How much salt is in a salt saturated solution?
Allow the diluent to evaporate and then weigh the remaining solute crystals
How do you make diluent for cell counter analyser?
sodium sulphate anhydrous 9.7 g/l Sodium chloride 4 g/l Dimethylolurea 0.4 - 1.0 g/L Buffered to pH 7.4
To prepare 300 ml of a 115 dilution from a 13 dilution you need to add ml of the 13 dilution to ml of diluent?
33,4ml
What dilution factor is 0.5 ml in 7.5 ml of diluent?
16
How do you do a serial dilution of a specific concentration of culture?
bacterial cell numbers needs reducing ,which is done by repeatedly diluting the amount of you have in your sample. A small amount of bacterial sample is mixed with a diluent solution(such as sterile broth), and then dilution are made. by adding small amount of diluted bacteria samples then spread onto the agar plate by L-shaped glass rod.
What would be the dilution if 96 mL of diluent is added to 4 ml of a bacterial suspension?
100
Why is a 20-gram sample of hamburger blended with 180 ml of diluent to yield a 110 dilution before making further dilutions?
20 grams is equivalent to 20ml. 20ml(sample) +180ml(diluent) = 200ml. 20ml is 10th part of 200ml. Ratio will yield a 1:10 dilution.
How much diluent do you need to add to a 2 gm vial of Rocephin to get a concentration of 250 mgml?
8 ml
How do you perform doubling dilutions?
Take a portion of that which you wish to dilute and put it in a vessel - e.g. a test tube. Add an equal portion of the diluent. You have just doubled the dilution. Take one portion of the mixed diuted liquid you have just made and put it in another tube and add the same volume of the diluent. The original liquid is now diluted again. The first tube contained a x2 dilution of the stuff, the 2nd tube contains a x4 dilution. If you carried this process on you would end up with x8, x16, x32 etc.etc. These are doubling dilutions. Hope this helps!
How much diluent is needed to make One-100th dilution using 4mL of serum?
To dilute 4 ml of serum by 100-fold, you need to make the final volume 400 ml. This may or may not be accomplished by adding 396 ml, depending on if the volumes of serum and diluent are additive or not.
Why it is important to add only one third of the diluent at first saving the remaining two-thirds until after the medication bottle has been vigorously shaken?
There are a few reasons why it is important to add only one third of the diluent to the medication bottle at first and save the remaining two-thirds until after the medication has been vigorously shaken. First, adding only a small amount of diluent at first allows you to better control the final volume of the solution. This is important because the final volume of the solution will determine the concentration of the medication, and it is important to ensure that the solution has the correct concentration. Second, adding only a small amount of diluent at first allows you to more easily mix the medication and diluent together. This is because adding all of the diluent at once can make it difficult to mix the two substances together thoroughly. By adding only a small amount of diluent at first and then shaking the bottle vigorously, you can ensure that the medication and diluent are well mixed. Finally, adding only a small amount of diluent at first can help to prevent the solution from foaming or frothing. This is because when a medication is mixed with a diluent, it can cause the solution to foam or froth, which can make it difficult to accurately measure the final volume of the solution. By adding only a small amount of diluent at first, you can help to prevent the solution from foaming or frothing, which can make it easier to accurately measure the final volume of the solution.
How do you prepare 1mM edta from 0.1M edta-Na2 solution?
You dilute it 1:10, then you take 1 part of that solution and mix it with 9 parts of the diluent. That will make the 1:100 dilution you need, incl. prevention of pipette inaccuracy.
What is the explanation for tenfold dilution technique?
In ten fold dilution we add one part of the sample into the nine part of the diluent e.g. water. It will make it ten fold dilute. If we have series of tubes to dilute then after making the ten fold dilution in first tube, take the dilute sample from the first tube in same quantity as we added sample in first tube and add it to 2nd one. then then take the same quantity from 2nd one and add to third one and so on......... from the last tube we take the adjusted quantity of dilute sample and discard it. This will make the series of ten fold dilution. If you add one part substance to 10 parts of water, you get an 11-fold dilution.
Why do you inject aspirated air from the syringe into the vital of diluent when you are reconstituting a drug for injection?
creates positive pressure that lets you draw the diluent easily. If you do not add air, a vacuum forms, making it difficult to draw the diluent.
