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If you have 0.05 ml of an undiluted culture at a concentration of 3.6x10 6 CFU ml You then add 4.95 ml sterile diluent What is the dilution factor and what is the final concentration of cells?
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∙ 13y ago
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∙ 13y ago
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What is a two-fold dilution?
A two-fold dilution involves taking a portion of a solution and mixing it with an equal volume of diluent, resulting in a solution that is half the concentration of the original. This process is often used in laboratories to decrease the concentration of a substance and make it suitable for further testing or analysis.
How do you do a serial dilution of a specific concentration of culture?
bacterial cell numbers needs reducing ,which is done by repeatedly diluting the amount of you have in your sample. A small amount of bacterial sample is mixed with a diluent solution(such as sterile broth), and then dilution are made. by adding small amount of diluted bacteria samples then spread onto the agar plate by L-shaped glass rod.
What is second dilution factor?
The second dilution factor refers to the factor by which a solution is further diluted after an initial dilution step. It is calculated by multiplying the volume of the original solution added to the new diluent by the volume of the new diluent divided by the final volume of the diluted solution.
What is the dilution of 10 ppm to 1 ppm?
To dilute a 10 ppm solution to 1 ppm, you would mix 1 part of the 10 ppm solution with 9 parts of a diluent (such as water). This would result in a 1 ppm concentration because the concentration has been decreased by a factor of 10 during the dilution process.
How do you dilute urine samples?
To dilute a urine sample, you can mix it with distilled water or a diluent solution in a clean container. The amount of diluent added will depend on the desired dilution ratio. Make sure to mix the sample thoroughly before testing.
Direct dilution vs double dilutions?
Direct dilution involves adding a specific volume of a sample directly to a diluent. Double dilution involves first diluting the sample in a known volume of diluent, then taking a portion of that dilution and further diluting it in a new volume of diluent. Double dilution is often carried out to reduce the concentration of a sample by a larger factor than with direct dilution.
To prepare 300 ml of a 115 dilution from a 13 dilution you need to add ml of the 13 dilution to ml of diluent?
33,4ml
What is a two-fold dilution?
A two-fold dilution involves taking a portion of a solution and mixing it with an equal volume of diluent, resulting in a solution that is half the concentration of the original. This process is often used in laboratories to decrease the concentration of a substance and make it suitable for further testing or analysis.
How do you do a serial dilution of a specific concentration of culture?
bacterial cell numbers needs reducing ,which is done by repeatedly diluting the amount of you have in your sample. A small amount of bacterial sample is mixed with a diluent solution(such as sterile broth), and then dilution are made. by adding small amount of diluted bacteria samples then spread onto the agar plate by L-shaped glass rod.
What is second dilution factor?
The second dilution factor refers to the factor by which a solution is further diluted after an initial dilution step. It is calculated by multiplying the volume of the original solution added to the new diluent by the volume of the new diluent divided by the final volume of the diluted solution.
What is the dilution of 10 ppm to 1 ppm?
To dilute a 10 ppm solution to 1 ppm, you would mix 1 part of the 10 ppm solution with 9 parts of a diluent (such as water). This would result in a 1 ppm concentration because the concentration has been decreased by a factor of 10 during the dilution process.
Why is a 20-gram sample of hamburger blended with 180 ml of diluent to yield a 110 dilution before making further dilutions?
The initial 110 dilution was made to decrease the concentration of the hamburger sample in order to make it easier to work with when making further dilutions. This initial dilution allows for a more accurate and precise measurement of the sample, ensuring that subsequent dilutions are consistent and reliable.
What would be the dilution if 96 mL of diluent is added to 4 ml of a bacterial suspension?
100
What does it means by 100 times dilution of stock dilution?
A 100 times dilution of a stock solution means that you are adding 1 part of the stock solution to 99 parts of a diluent (such as water) to make a total volume of 100 parts. This results in a final solution that is 1/100th the concentration of the original stock solution.
What dilution factor is 0.5 ml in 7.5 ml of diluent?
The dilution factor is 1:15. This is calculated as the total volume (7.5 ml) divided by the volume of the sample (0.5 ml).
What is the explanation for tenfold dilution technique?
Tenfold dilution technique involves mixing one part of a solution with nine parts of a diluent, resulting in a tenfold decrease in concentration. This is commonly used in laboratories to create a series of solutions with decreasing concentrations for analysis. Each successive dilution results in a further tenfold decrease in concentration.
How do you dilute urine samples?
To dilute a urine sample, you can mix it with distilled water or a diluent solution in a clean container. The amount of diluent added will depend on the desired dilution ratio. Make sure to mix the sample thoroughly before testing.
