0.1 % Triton X-100 is a 1 in 1000 dilution of 100% Triton X-100 (available for purchase). For example, add 1ml 100% Trition to 999ml water to yield 0.1% Triton. I usually keep a stock of 10% triton on hand to make various buffers.
take 1 ml, add 9 ml water.
1 part of solution A plus 99 parts solution B
HPUS is: The Homœopathic Pharmacopœia of the United States 2X means 1:100 dilution. In other words 1%. Normally homeopathic dilutions are much more. 12X or 600X.
Add 2 mL of culture to 20 mL of buffer. 2/20 = 1/10
A 100 times dilution of a stock solution means that you are adding 1 part of the stock solution to 99 parts of a diluent (such as water) to make a total volume of 100 parts. This results in a final solution that is 1/100th the concentration of the original stock solution.
An 8-fold dilution means you are making your solution 1/8 less concentrated. So, if you want to make 100 ml of a solution diluted 8 fold you would do as follows: 1/8=.125 100*.125= 12.5 Mix 12.5 mL of your concentrated solution with 87.5 mL of your solvent (water etc..) to bring the final volume to 100 mL. If you wanted to perform a ten fold dilution you would simply mix 10 mL of your concentrated solution with 90 mL of your solvent. 1/10=.1 100*.1=10
You add 9.09ml of stock solution to a volumetric and make it up to 1 litre to get a 110 dilution
Dilution is the process of reducing the concentration of a substance by adding more solvent. The dilution factor is the ratio of the final volume of the diluted solution to the initial volume of the concentrated solution. It is used to calculate the new concentration after dilution.
To make a 1 to 5 dilution you mix 1 part of your substance with 4 parts water. ie: Mix 1 tablespoon of creamer with 4 tables spoons of coffee, and the coffee is 1/5 creamer now.
100
To find out how many ounces of chemical it would take to make 100 gallons of finished dilution at 0.5%, we can set up a proportion. Since 11 ounces of chemical are used in 10 gallons of water, we can establish the ratio of ounces of chemical to gallons of water as 11:10. By setting up a proportion and solving for x, we find that it would take 110 ounces of chemical to make 100 gallons of finished dilution at 0.5%.
0.1 % Triton X-100 is a 1 in 1000 dilution of 100% Triton X-100 (available for purchase). For example, add 1ml 100% Trition to 999ml water to yield 0.1% Triton. I usually keep a stock of 10% triton on hand to make various buffers.
To make a 500 dilution, add 1 part of the substance you are diluting to 499 parts of water. For example, if you have 1 mL of the substance, you would add 499 mL of water to make a total volume of 500 mL for the dilution.
You dilute it 1:10, then you take 1 part of that solution and mix it with 9 parts of the diluent. That will make the 1:100 dilution you need, incl. prevention of pipette inaccuracy.
take 1 ml, add 9 ml water.
To make a 50% acetone control, you can mix equal parts of acetone and water. For example, if you start with 10 ml of acetone, you would add 10 ml of water to make a 50% acetone solution.