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The Beer-Lambert Law:
A = epsilon*b*c
A is absorbance (unitless)
epsilon is the extinction coefficient at a particular wavelength (L cm-1 mol-1)
b is the path length of the cuvette (cm)
c is the concentration of the solution (mol/L)
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Is it possible to calculate the chromacity coordinates using absorbance values?
Yes, it is possible to calculate the chromaticity coordinates using absorbance values. The best way to calculate the chromaticity coordinates using absorbance values is by using the formula x = x/x+y+z.
What is a equation that is the inverse of the exponential equation?
Logarithmic equation
What is x in this equation?
what is the equation
What is the equation of this question?
The equation is "What = ?"
How do you put equation in a sentence?
"Figure out this mathematical equation" "This is how to figure out an equation" "An equation is something widely used in mathematics."
Is there a simple equation to calculate the concentration given the absorbance and a time?
You need a graphic concentration versus absorbance.
Is wavelength or absorbance the dependent variable?
"absorbance"Since in the experiment, you probably choose the wavelength, then measure the absorbance (absorption?, the absorbance is the dependent variable.
What is the purpose of the blank?
Blank Sample in Spectrophotometry is used to measure the absorbance of light without sample. It is subtracted from the total absorbance for measurement of Absorbance from a sample's absorbance.
What is the purpose of blank samples?
Blank Sample in Spectrophotometry is used to measure the absorbance of light without sample. It is subtracted from the total absorbance for measurement of Absorbance from a sample's absorbance.
What is difference between specific absorbance and absorbance interms of spectroscopy?
specific absorbance- it is absorbance in a solution containing one gm of substance in 100 ml solvent in 1cm shell. so it is having a difference with absorbance which is negative logarithm of incident light to the transmitted light. divya.chakraborty@gmail.com
What is the different between primary absorbance and secondary absorbance in fluorescence?
in primary light absorbed by outer molecule while in secondary re-absorbance occurs
Why is absorbance plotted instead of percent transmission when following bacterial growth?
Because when we read absorbance, it's the amount of light absorbed by the bacteria itself. Absorbance is directly related to the amount of bacteria. More absorbance = more bacteria.
What is the specific absorbance of aspirin?
If you have a spectrofotometer ( the thing to mesure the absorbance) then play with the setting and use a maximum. this will lay close to your specific absorbance or take the pharmacopea or a MERCK index
What is the abbreviation for Absorbance?
A
Why are the absorbance values obtained from the conversion chart rather than the absorbance scale on the spectronic 20?
because that chart gives a more accurate value than the absorbance scale on the specthometor
What are two reasons why absorbance measurements are taken at lambda max?
to ensure maximum absorbance of light by the solution
What is external calibration and is it the same as the manual calibration of equipment?
Well, external calibration is a method used in analytical chemistry to determine the concentration of an unknown analyte. In essence, you take known concentrations of the analyte and plot it on an absorbance or transmittance graph to get a linear plot. And then you take that linear equation and plug in the absorbance or transmittance value received from the unknown solution and get the concentration. An example of this is if you want to find out the amount of calcium in a vitamin tablet. Dissolve the vitamin tablet and test the solution to get an absorbance value. Then test by the same method various concentrations of a calcium solution, plot this on a graph of absorbance vs. concentration and there yah go.
