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How can you calculate absorbance of unknown from linear equation?
To calculate the absorbance of an unknown sample using a linear equation, you first need to establish a calibration curve by plotting the absorbance values of known standards against their concentrations. The resulting linear equation, typically in the form (y = mx + b), relates absorbance (y) to concentration (x), where (m) is the slope and (b) is the y-intercept. By measuring the absorbance of the unknown sample and substituting this value into the linear equation, you can solve for the concentration of the unknown sample. This allows you to determine the absorbance based on its concentration derived from the calibration curve.
What is maximum absorbance of methyle orange at 242 nm?
The maximum absorbance of methyl orange typically occurs at around 464 nm, not 242 nm. At 242 nm, the absorbance may be lower or not significant, as this wavelength is outside the main absorption range for methyl orange. For accurate absorbance values, it is important to refer to specific absorption spectra or experimental data for methyl orange.
Is it possible to calculate the chromacity coordinates using absorbance values?
Yes, it is possible to calculate the chromaticity coordinates using absorbance values. The best way to calculate the chromaticity coordinates using absorbance values is by using the formula x = x/x+y+z.
Why do protein have 2 absorbance peak at 280 nm?
Proteins exhibit two absorbance peaks around 280 nm primarily due to the presence of aromatic amino acids, such as tryptophan and tyrosine. Tryptophan has a strong absorbance peak near 280 nm, while tyrosine contributes a smaller peak at the same wavelength. The combined absorbance from these amino acids allows for the estimation of protein concentration in solutions, as they are key components in the protein structure.
What does the negative absorbance show?
Negative absorbance values typically indicate that the measured sample has lower light absorption than the baseline or reference. This can occur due to factors such as instrument noise, incorrect baseline correction, or interference from other substances. In some cases, it may suggest the presence of scattering or fluorescence rather than true absorbance. Negative absorbance values should be interpreted cautiously and may require further investigation to clarify the underlying cause.
Is there a simple equation to calculate the concentration given the absorbance and a time?
You need a graphic concentration versus absorbance.
Is wavelength or absorbance the dependent variable?
"absorbance"Since in the experiment, you probably choose the wavelength, then measure the absorbance (absorption?, the absorbance is the dependent variable.
What is the purpose of the blank?
Blank Sample in Spectrophotometry is used to measure the absorbance of light without sample. It is subtracted from the total absorbance for measurement of Absorbance from a sample's absorbance.
What is difference between specific absorbance and absorbance interms of spectroscopy?
specific absorbance- it is absorbance in a solution containing one gm of substance in 100 ml solvent in 1cm shell. so it is having a difference with absorbance which is negative logarithm of incident light to the transmitted light. divya.chakraborty@gmail.com
Is absorbance considered a discrete or continuous variable?
Absorbance is considered a continuous variable.
What is the different between primary absorbance and secondary absorbance in fluorescence?
in primary light absorbed by outer molecule while in secondary re-absorbance occurs
What is the specific absorbance of aspirin?
If you have a spectrofotometer ( the thing to mesure the absorbance) then play with the setting and use a maximum. this will lay close to your specific absorbance or take the pharmacopea or a MERCK index
The absorbance of a solution of NiCl2 of unknown concentration is found to be 0.388 what is the concentration of this soultion?
The concentration of the NiCl2 solution can be determined by using Beer's Law, which states that absorbance is directly proportional to concentration. You would need to know the molar absorptivity of NiCl2 at that specific wavelength in order to calculate the concentration. Plugging in the values of absorbance and molar absorptivity into Beer's Law equation would give you the concentration of the NiCl2 solution.
What is the abbreviation for Absorbance?
A
What is baseline in uv?
In UV spectroscopy, the baseline refers to the horizontal line at zero absorbance on the absorbance axis. It represents the reference point for measuring the absorbance of the sample. The baseline should be stable and noise-free to ensure accurate measurement of the absorbance of the sample.
What is the relationship between the optical density (OD) and absorbance in spectrophotometry?
In spectrophotometry, optical density (OD) and absorbance are directly related. As the OD increases, the absorbance also increases. This means that a higher OD value indicates a higher absorbance of light by the sample being measured.
Why are the absorbance values obtained from the conversion chart rather than the absorbance scale on the spectronic 20?
because that chart gives a more accurate value than the absorbance scale on the specthometor
