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The Beer-Lambert Law:
A = epsilon*b*c
A is absorbance (unitless)
epsilon is the extinction coefficient at a particular wavelength (L cm-1 mol-1)
b is the path length of the cuvette (cm)
c is the concentration of the solution (mol/L)
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Is it possible to calculate the chromacity coordinates using absorbance values?
Yes, it is possible to calculate the chromaticity coordinates using absorbance values. The best way to calculate the chromaticity coordinates using absorbance values is by using the formula x = x/x+y+z.
What is a equation that is the inverse of the exponential equation?
Logarithmic equation
How do you know if a value a is a solution to an equation?
If this value a satisfy the equation, then a is a solution for that equation. ( or we can say that for the value a the equation is true)
What is the term used for a quantity which when substituted for the unknown quantity in an equation satisfies the equation?
It is the solution to the equation or a root of the equation.
How do you put equation in a sentence?
"Figure out this mathematical equation" "This is how to figure out an equation" "An equation is something widely used in mathematics."
Is there a simple equation to calculate the concentration given the absorbance and a time?
You need a graphic concentration versus absorbance.
Is wavelength or absorbance the dependent variable?
"absorbance"Since in the experiment, you probably choose the wavelength, then measure the absorbance (absorption?, the absorbance is the dependent variable.
What is the purpose of the blank?
Blank Sample in Spectrophotometry is used to measure the absorbance of light without sample. It is subtracted from the total absorbance for measurement of Absorbance from a sample's absorbance.
What is difference between specific absorbance and absorbance interms of spectroscopy?
specific absorbance- it is absorbance in a solution containing one gm of substance in 100 ml solvent in 1cm shell. so it is having a difference with absorbance which is negative logarithm of incident light to the transmitted light. divya.chakraborty@gmail.com
The absorbance of a solution of NiCl2 of unknown concentration is found to be 0.388 what is the concentration of this soultion?
The concentration of the NiCl2 solution can be determined by using Beer's Law, which states that absorbance is directly proportional to concentration. You would need to know the molar absorptivity of NiCl2 at that specific wavelength in order to calculate the concentration. Plugging in the values of absorbance and molar absorptivity into Beer's Law equation would give you the concentration of the NiCl2 solution.
What is the different between primary absorbance and secondary absorbance in fluorescence?
in primary light absorbed by outer molecule while in secondary re-absorbance occurs
What is the specific absorbance of aspirin?
The specific absorbance of a substance like aspirin refers to its unique ability to absorb light at a specific wavelength. To find the specific absorbance of aspirin, you would need to measure its absorbance at a specific wavelength using a spectrophotometer.
What is baseline in uv?
In UV spectroscopy, the baseline refers to the horizontal line at zero absorbance on the absorbance axis. It represents the reference point for measuring the absorbance of the sample. The baseline should be stable and noise-free to ensure accurate measurement of the absorbance of the sample.
What is the abbreviation for Absorbance?
A
Why are the absorbance values obtained from the conversion chart rather than the absorbance scale on the spectronic 20?
because that chart gives a more accurate value than the absorbance scale on the specthometor
Is absorbance and OD the same thing or any difference?
Absorbance and optical density (OD) are often used interchangeably to describe the light absorbance properties of a substance. However, technically, absorbance refers to the logarithm of the ratio of the intensity of light incident on a material to the intensity transmitted through the material, while OD specifically refers to the absorbance measured in a spectrophotometer at a specific wavelength.
What wavelength is the peak absorbance for cobalt chloride?
The peak absorbance for cobalt chloride typically occurs around 550-600 nm.
