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If you added more glucose that the Kliger Iron Agar test than is called for you risk getting false results. The bacteria need to exhaust the glucose to turn the solution red or yellow. If too much glucose is present you may not be able to determine if the bacteria can ferment animal proteins.

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Q: What would happen if 1 percent glucose is added rather than the amount specified in the recipe for the KIA?
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What will happen when red blood cells are kept in 10 percent glucose solution?

nothing


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