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If you added more glucose that the Kliger Iron Agar test than is called for you risk getting false results. The bacteria need to exhaust the glucose to turn the solution red or yellow. If too much glucose is present you may not be able to determine if the bacteria can ferment animal proteins.

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Suppose that a scientist is simultaneously measuring both the amount of oxygen and the amount of glucose that is being used by cells. If a chemical were added that inhibited the electron transport chain?

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