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DNA is best stored at 4 degrees Celsius because anything colder may cause extensive single and double strand breaks.
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What is the function of solution 2 in plasmid isolation?
This solution contains the detergent sodium dodecyl sulfate (SDS) which dissolves the cell membrane and denatures proteins. The solution is very alkaline (pH > 12) due to the presence of sodium hydroxide. The high pH aids in denaturing proteins and causes the cleavage of the phosphate bonds in RNA. This eliminates interference from high molecular weight RNA during the plasmid purification. Under highly alkaline conditions, the two strands in non-supercoiled DNA (linear fragments of chromosomal DNA, relaxed and nicked circular DNA) separate and are partially removed from solution. However, this does not occur with supercoiled forms of plasmid DNA because the two strands are intertwined and entangled in a way that prevents them from coming apart. Therefore, supercoiled plasmid remains free in solution.
What is the function of the chromatain?
The chromatain have four major functions. They package DNA into a smaller volume to fit in the cell. They strengthen the DNA to allow mitosis, and they prevent damage to DNA. Chromatain control gene expression and DNA replication.
What are four charactoristics of eubacteria?
eubacteria lack a nucleus, lack histones in their DNA, have no membrane bound organelles, and their DNA is in a circular form.
Describe the function of electricity and the agarose gel in electrophoresis?
The electricity pulls the polar DNA strands through the gel, and shorter DNA strands move farther because they are less inhibited by the gel. The gel acts as drag to separate the different length DNA strands, so different DNA creates specific dye bands.
What is the function of primers?
RNA primers are used to initiate the DNA replication at the template strand. DNA molecules require a free 3' OH, to which it could add the nucleotides. This free 3' OH is provided by the RNA primer. So prior to the synthesis of DNA a short fragment of RNA is synthesized that is later excised and filled with DNA molecules.
What are plasmid genes?
A plasmid is an extra chromosomal DNA molecule separate from the chromosomal DNA which is capable of replicating independently from the chromosomal DNA http://en.wikipedia.org/wiki/Plasmid I think this is far use.
What is extrachromosomal replicon?
+other than chromosomal DNA is known as extra chromosomal DNA like plasmids, mitochondria and chloroplast containing DNA.
The chromosomal DNA is duplicated in?
nucleus
What enzyme inserts viral DNA into the host's chromosomal DNA?
integrase
Circular extra genomic DNA is called?
A circular band of DNA that exists separate of chromosomal DNA is called a plasmid. Plasmids reside within bacteria cells and can replicate independently of the chromosomal DNA.
Can a bacteraial plasmid replicate independently of the bacterial chromosome?
Bacteria carry plasmids which is a double stranded DNA . It is how their extra chromosomal DNA is stored, they also have chromosomal DNA.Plasmids are extra-chromosomal DNA in Bacterial cells that replicate independently in cell. Plasmids are ubiquitous- means significant number of bacteria have plasmid and can have more that one plasmids. But Plasmids do not occur in all bacterial isolates.
What is Chromosomal Theory?
DNA is stored on chromosomes, and chromosomes are passed through generations.
How are chromosomal DNA and mitochondrial DNA inherited differently?
Chromosomal DNA is inherited from both parents via the egg and the sperm. Since Spermatozoa do not any Mitochondria you get all of your Mitochondria DNA from the Egg. IE your Mum.
What is the difference between plasmid and episomes?
Plasmid DNA is an extra-chromosomal DNA molecule, it cannot link up with chromosomal DNA, and it contains the genetic informations that are necessary for its own replication. Episomes is any kind of extra-chromosomal DNA that can link up with chromosomal DNA. That is the main difference between them two. Episomes are usually larger than other extra-chromosomal DNA. An example of episome are the viruses, because they intergrate their genetic material into the host's chromosomal DNA.The only difference between the plasmid and the episome is the integration i-e plasmid can not integrate while episome can integrate into the genome.
What process in bacteria always allow both chromosomal and non-chromosomal DNA to be transferred?
Binary fission
What is a part of a cell that contains the chromosomal DNA?
The Nucleus
The chromosomal DNA is duplicated in what?
I think the nucleus is the best answer.
