Your question is unanswerable because we have no idea what you're connecting. Without further information as to what these wires are for I would say leave the wires to a professional.
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No, not without a load being between them. A direct connection will create a short circuit and the circuit protection will open the circuit and stop the current flow.
Assuming the wires are the correct gauge for application and breaker you use black and white wires as hot. Put red electrical tape on each end of white wire and connect red and black to the breaker output and bare wire to ground lug in panel. At receptacle connect black and red to hot contacts and bare wire to ground lug.
There is 220 volts between the two poles. If you are running 2 wires (black and white) + ground then you hook black to one pole and white to the other. Put red or black electric tape on each end of the white wire and wrap around wire for 3 inches or so next to the connection so the next person will be able to see that the wire is hot and not a neutral.
The neutral wire and power wire are never connected together.
If you are referring to lamp cord type wire where both wires are brown then yes, connect the wire with the groves to the white neutral and the smooth wire to the black hot wire.
Red is hot Green is ground White is neutral