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Yes. Electrons always flow from the negative pole to the positive pole. In a Direct current(DC) circuit, one pole is always negative, the other pole is always positive and the electrons flow in one direction only. In an alternating Current (AC) circuit the two poles alternate between negative and positive and the direction of the electron flow continually reverses. In DC circuits (e.g. for a battery) positive is usually marked red and negative is usually marked black, but other color schemes are very common in automotive and telecommunications systems. In AC systems which are at no point connected to earth (unlike domestic mains wiring where the neutral is commonly earthed at substations and generators) polarity is not important as the two wires alternate polarity many times per second.
LED's are DC voltage. Transformers are AC voltage. There is no positive or negative on AC voltage. You would need a diode to change the AC to DC, then there would be positive and negative voltages.
A 220 vac circuit has 2 hot wires and a neutral. The neutral stays at 0 volts and the hot wires vary between positive and negative. When one is positive, the other is negative.
In a DC circuit it is positive. In a 220v ac circuit it's one of the two positive (hot) lines.
If a,b, and c are positive a < x < b means ax < cx If a,b , and c are negative a < x < b means ax > cx