Usually, yes. Obviously, only if you have one: the two variables could be inter-dependent.
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.... then your graph is inverted.
It depends on which variable is independent, and which one is dependent (its value is determined by a function of the independent variable). So suppose that concentration is a function of an arbitrary length (the length is what determines concentration). The independent variable (length) is put on the horizontal axis, and the dependent variable (concentration) is put on the vertical axis.
The independent variable.
the dependent variable changes with the independent variable. the independent variable only changes when changed by the experimenter. Time is usually an independent variable.
An independent variable is a factor that doesn't rely on anything, unlike the dependent variable (which relies on the independent variable). The most common independent variable is time.