take 1 ml, add 9 ml water.
To make a 1 to 100 dilution, start by taking 1 part of the concentrated solution and add it to 99 parts of a diluent (usually water or a buffer). For example, if you use 1 mL of the concentrated solution, mix it with 99 mL of the diluent. Ensure thorough mixing to achieve a uniform dilution. This results in a final solution that is 1% of the original concentration.
The wording is important here. If it is a 2 *in* 5 dilution, then the scientist would need 200 ml of concentrate. The best way would be to take a graduated cylinder, put about 200 ml of solvent into the cylinder. Then put in the 200 ml of concentrate. Then add enough additional solvent to make a total of 500 ml. Mix, then pour it into a labeled and dated bottle, and screw on the lid. If it is a 2 *to* 5 dilution, then you need 2/7ths concentrate, and 5/7ths solvent. One seventh of 500 ml is about 71.43, so you would need 142.86 ml of concentrate, and fill the rest with solvent until the total is 500 ml.
To make a 1 to 1 dilution, you combine equal volumes of the concentrated solution and a diluent, typically a solvent like water. For example, if you have 10 mL of the concentrated solution, you would add 10 mL of the diluent. Mix thoroughly to ensure a uniform solution. This results in a final volume of 20 mL at half the concentration of the original solution.
Add 2 mL of culture to 20 mL of buffer. 2/20 = 1/10
33,4ml
take 1 ml, add 9 ml water.
To make a 500 dilution, add 1 part of the substance you are diluting to 499 parts of water. For example, if you have 1 mL of the substance, you would add 499 mL of water to make a total volume of 500 mL for the dilution.
1 in 25. (10/250)
32
measure 1 ml of the original solution and add 6.6 ml of distilled water.
When 0.25 ml is diluted to a final volume of 20 ml, the resulting dilution can be calculated as the ratio of the original volume to the final volume. This is calculated as 0.25 ml / 20 ml, which simplifies to a dilution factor of 1:80. Therefore, the resulting dilution is 1:80.
The dilution factor is 1:15. This is calculated as the total volume (7.5 ml) divided by the volume of the sample (0.5 ml).
To make a 50% acetone control, you can mix equal parts of acetone and water. For example, if you start with 10 ml of acetone, you would add 10 ml of water to make a 50% acetone solution.
For all dilution/ concentration problems you use the simple equation: M1V1 = M2V2 2.40*V2 = 8.25*25 V2 = (8.25*25)/2.40 V2 = 85.9mL Final volume will be 86mL.
To make a 1 to 100 dilution, start by taking 1 part of the concentrated solution and add it to 99 parts of a diluent (usually water or a buffer). For example, if you use 1 mL of the concentrated solution, mix it with 99 mL of the diluent. Ensure thorough mixing to achieve a uniform dilution. This results in a final solution that is 1% of the original concentration.
The dilution factor is 1:100, as you're adding 4.95 ml to the original 0.05 ml. The final concentration is calculated by multiplying the original concentration by the dilution factor, resulting in a final concentration of 3.6x10^4 CFU/ml.