Yes, they did.
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the concept of the + sign. they also developed the concept of zero. and the+ sign
It is attributed to Indian mathematicians.
The ancient Mayans were skilled in math and developed the concept of zero. They had a highly advanced number system and were able to perform complex mathematical calculations. This development of zero greatly influenced mathematics throughout the world.
Gupta mathematicians developed the concept of zero in the use of math, and also developed the decimal system based on the number 10. They also created a number writing system that was later adopted by the Islamic Empire. This system became known as Arabic Numerals, but is really a Gupta achievement.
They started in the Indian sub-continent thousands of years ago when the concept of zero was first realised.