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Is reverse-transcription polymerase chain reaction quantitative or qualitative?
Certainly rt-PCR is qualitative and can also theoretically be quantitative. Anneal the RNA to get a 1:1 RNA to DNA copy, then proceed with quantitative PCR.
What is difference between Qualitative PCR and Quatitative PCR?
In qualitative PCR specific DNA fragment is detected while in quantitative PCR our target DNA sequence not only is detected but its amount is determined (after reaction we can calculate the amount of DNA we had in our sample)
Differentiate between quantitative and real time PCR?
: Differentiate between quantitative and real time PCR.
What is quantitative pcr technology used for?
Quantitative PCR Technology is used in biochemistry, in particular molecular biology. The PCR stands for polymerase chain reaction and is used to "amplify" pieces of DNA to make millions of copies of a particular DNA strand.
What is molecular assays?
Molecular assays are laboratory techniques that detect and analyze the genetic material (DNA or RNA) of organisms. These assays are used to identify specific genes, mutations, or pathogens, and are widely used in research, diagnostics, and pharmaceutical development. Examples of molecular assays include polymerase chain reaction (PCR), next-generation sequencing (NGS), and hybridization assays.
How long has real time pcr been around?
Real-time PCR, also known as quantitative PCR (qPCR), has been around since the mid-1990s. It gained popularity for its ability to monitor the amplification of DNA during the PCR process in real time, providing quantitative data on DNA or RNA targets.
HBV Quantitative real time PCR blood?
Quantitative real-time PCR for Hepatitis B Virus (HBV) measures the amount of viral DNA in a blood sample. This test is used to monitor the levels of HBV in patients undergoing treatment and to assess disease progression and response to therapy. It helps healthcare providers determine the stage of infection and make treatment decisions.
What is pcr and types of pcr?
PCR (polymerase chain reaction) is a molecular biology technique used to amplify a specific segment of DNA. There are various types of PCR, including quantitative PCR (qPCR) for quantification of DNA, reverse transcription PCR (RT-PCR) to amplify RNA, nested PCR for increased specificity, and digital PCR for absolute quantification of nucleic acids.
What are the differences between conventional pcr andreal time pcr?
PCR allows amplification of DNA for a specific gene, after too many cycles of PCR the result will reach saturation, basically meaning all of the DNA has been amplified. Conventional PCR will basically tell you whether or not a gene is expressed in your sample. This can be done semi-quantitavely if the PCR is performed for a low number of cycles, ie it will tell you whether one sample expresses more of your gene of interest than another sample. The results are seen by separating the PCR products by agarose gel/ethidium bromide electrophoresis. Real-time PCR will record exactly what cycle of PCR a detectable level of amplified product became detectable, giving a far more accurately quantifiable estimation of gene expression.
What are the pros and cons of RT-PCR?
Pros: RT-PCR is highly sensitive and specific, allowing for detection and quantification of RNA targets. It is widely used for diagnosing infectious diseases, monitoring gene expression, and detecting genetic mutations. Cons: RT-PCR requires specialized equipment and trained personnel, making it relatively costly and less accessible in resource-limited settings. There is also a potential for contamination during the amplification process, leading to false-positive results.
1)Human immunodeficiencyvirus contains an RNA as its genetic material.Between standard PCR and RT PCR techniques, which one would you employ to amplify a desired gene from this RNA virus.Explain how you would monitor disease progression and therapy?
You would employ reverse transcription PCR (RT-PCR) to amplify a desired gene from an RNA virus like human immunodeficiency virus (HIV) because RT-PCR can convert the RNA into complementary DNA (cDNA) before amplification, making it suitable for RNA viruses. To monitor disease progression and therapy in HIV, you would measure viral load by quantifying the amount of viral RNA in the blood using techniques like quantitative PCR or real-time RT-PCR. Additionally, you could monitor immune function by measuring CD4 cell counts to assess the impact of antiretroviral therapy and disease progression.
What are the benefits of using the TaqMan Gene Expression Assays?
TaqMan Gene Expression Assays consist of a pair of unlabeled PCR primers and a TaqMan probe with a FAM or VIC dye label on the 5' end, and minor groove binder (MGB) nonfluorescent quencher (NFQ) on the 3' end.
