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I couldn't tell you what were the odds, but I hope this helps: You could be killing your oxidase activity if your organism was no longer viable. When identifying organisms you should always use fresh cultures. Regardless, some strains of Pseudomonas aeruginosa can test oxidase negative. a TSI slant would confirm if you were dealing with a presumptive pseudomonad or an enteric. Enterics will have a strong acid reaction (because they ferment) and Pseudomonas would have no acid (or a very weak acid) reaction. Happy streaking!

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Q: Pseudomonas Aeruginosa is generally oxidase positive if the organism were refrigerated for an extended period is there a chance that it would produce a negative oxidase reaction?
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