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a biased sample is valid determin
Because without representative sample, your results will not be valid.
Providing of course that a sample is representative of the population from which it is drawn, the bigger it is the more likely it will be to lead to a valid conclusion. Therefore, the best sample size when there are no restrictions, as in this case, would be one of 1000.
Well, if your sample does not represent the larger sample, you'll certainly not get a valid result ... For example, if you're studying pregnancy and your sample includes men - The whole idea of "representative' sample is fuzzy and often gives interesting errors.
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