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Jarret and Trieste (1987) outline a few assumptions but the basic one is 'uniform flow'. Meaning the flow does not vary throughout the water body.

Other ideas are that n might not be valid over slopes with 0.04m/m gradients because shear stress from the channel will no longer be the dominant source of resistance.

Read Stream Hydrology: an introduction for ecologists, some pages available online

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Q: What are the assumptions for deriving Manning's equation for open channel flows?
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