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If you mean in Excel, yes: you must always start a formula with an equal sign.
In mathematics, such expressions indicate that the value of the expression to the left of the equal sign has the same value as the expression to the right of the equal sign. In some cases, it can also be interpreted to mean that the expression on one side of the equal sign can be used in place of the expression on the other side of the equal sign (say in manipulating algebraic expressions). In computer languages, the equal sign is sometimes also used to indicate that the value of the expression to the right of the equal sign is to be transferred to the location indicated by the expression to the left of the equal sign. The expression to the left of the equal sign is usually a single variable that represents a memory location.
A formula with an equal sign
In Algerbra, an expression is a math sentence, such as 5y+3x, that does not have an equal sign. When you include the equal sign it becomes equation.
Equals sign demonstrates that something is the same as something, or equal to.