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In assays, or medical tests, both qualitative and quantitative tests occur. The qualitative test identifies the presence or absence of the pathogen or toxin (or other molecule) being tested for, while the quantitative test hopes to measure how much of that particular substance is in the body.
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What is a four point biological assay?
Four point assay refers to an estimate of the potency of a drug by measuring the response it produces in a biological it produces i n abiological preparation
How do you calculate the assay percent in chemicals?
4.4 g of NaOH
What are standard assay conditions?
A standard assay is an analytic procedure for measuring the presence or amount or activity of the analyte. The general steps are; sample processing, target specific identification, target amplification system, and detection. detection.
What is the formula to calculate percent assay?
Final outcome ÷ possible outcome X100 (eg. If I got 23 out of 30 for a test then ---> 23÷30 X100 = 76.66= 77% ) hope this helped... :]*
How do you make a standard curve graph?
Standard curves are used to determine the concentration of substances. First you perform an assay with various known concentrations of a substance you are trying to measure. The response might be optical density, luminescence, fluorescence, radioactivity or something else. Graph these data to make a standard curve - concentration on the X axis, and assay measurement on the Y axis. Also perform the same assay with your unknown samples. You want to know the concentration of the substance in each of these unknown samples. To analyze the data, fit a line or curve through the standards. For each unknown, read across the graph from the spot on the Y-axis that corresponds to the assay measurement of the unknown until you intersect the standard curve. Read down the graph until you intersect the X-axis. The concentration of substance in the unknown sample is the value on the X-axis. In the example below, the unknown sample had 1208 counts per minute, so the concentration of the hormone is 0.236 micromolar. Prism makes it very easy to fit your standard curve, and to read (interpolate) the concentration of unknown samples.
What is assay for tablets?
Assay is done for qualitative and quantitative estimation of the content and amount of substance in the product . the product can be either raw material or final product.
What is the difference between assay and analysis?
Assay is the quantitative determination of the amount of a component in a sample. Purity is the determination of the impurities in the sample. It can be quantitative, semi-quantitative or qualitative. For example : if you dissolve 4 grams of compound A (with no impurities in it) in 6 grams of water, you get a solution of compound A with 100% purity and 40% assay. From the ICH Guideline Q2A (Validation of Analytical methods) : "- Testing for impurities can be either a quantitative test or a limit test for the impurity in a sample. Either test is intended to accurately reflect the purity characteristics of the sample. [...] - Assay procedures are intended to measure the analyte present in a given sample. In the context of this document, the assay represents a quantitative measurement of the major component(s) in the drug substance."
What is the difference between potency and assay?
Potency is the purity of the compound ;this states % available of the main component without water and residual solvents Assay is defined as Potency plus water and residual solvents and other remaining fractions please note assay can also be expressed as as is basis or dry basis Assay and Potency are same if assay is exprtessed as as is basis please note Potency word is mainly used for Antibiotics and bilogicals courtsey ; CDER , USFDA ; Dr.Uttam Krishna
What is the difference between assay on as is basis and assay on anhydrous basis?
Assay on as is basis refers to the percentage of a substance in its current state, including any impurities or water content present. Assay on anhydrous basis, on the other hand, refers to the percentage of a substance calculated without including any water content present. This difference is important when comparing the purity of substances or calculating accurate concentrations in chemical analysis.
Can heat coagulation be use to detect the presence of albumin in urine?
Yes, heat coagulation can be used to detect the presence of albumin in urine. When urine is heated, if albumin is present in significant amounts, it will coagulate and form a white precipitate. This method can provide a qualitative indication of the presence of albumin, but it is not as sensitive or specific as other laboratory tests like a urine dipstick or a quantitative albumin assay.
Difference between assay as is basis and dried basis?
Assay on 'as is' basis refers to the composition of a substance including its water content, while assay on 'dried' basis refers to the composition of a substance after removing the water content. Comparing these two types of assays can help in understanding the true chemical composition of a substance excluding the influence of water content.
How can you check or analyze the assay of Sodium tripolyphosphate?
You can analyze the assay of Sodium tripolyphosphate by conducting a quantitative chemical analysis using methods like titration or spectrophotometry. These methods involve measuring the concentration of the compound in a sample to determine its purity or assay level. Alternatively, you can also send the sample to a laboratory that specializes in analyzing chemical compounds for accurate results.
Why tartaric assay method falls under alkalimetric direct titration method?
The tartaric assay method falls under the alkalimetric direct titration method because it involves the direct titration of tartaric acid with a standardized alkali solution to determine its concentration. The method is based on the neutralization reaction between the tartaric acid and the alkali, which allows for the quantitative determination of the tartaric acid content present in the sample.
What is an assay pound?
An assay pound is a small standard weight used in assaying bullion, which varies between assayers, but is generally around half a gram.
Fiducial Limits in Microbiological Assay?
Fiducial limits in a microbiological assay represent the confidence interval within which the true value of an analyte is expected to fall. They are calculated based on the variability of the assay and provide a range in which the true value is likely to lie with a certain level of confidence. Fiducial limits are useful for assessing the precision and accuracy of an assay.
What is protein assay?
Protein assay is the determination of concentration or total level of protein in a solution.There are various protein assays employed like bradford assay and lowry assay
What is difference between end point and kinetic assay in biochemistry?
In biochemistry, an endpoint assay measures the final amount of a product formed or substrate remaining after a reaction has stopped, providing a single data point. A kinetic assay, on the other hand, measures the rate of a reaction by recording multiple data points over time. Kinetic assays provide additional information about reaction mechanisms, enzyme activity, and inhibitor kinetics compared to endpoint assays.
