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Q: What is the sequence of the primer 5' -ACGTGCT-3'?
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When a rifle fires a sequence of events takes place. After the firing pin strikes the primer what is the next event in the sequence?

When a rifle fires, a sequence of events takes place. After the firing pin strikes the primer, what is the next event in the sequence


When a rifle fires, a sequence of events takes place. After the firing pin strikes the primer, what is the next event in the sequence?

The primer explodes and ignites the gunpowder.


Nucleotide sequence that starts PCR processes?

Primer sequences


How can I check am I using correct primer sequence in my PCR?

You can check the correctness of your primer sequence by performing a basic sequence alignment using bioinformatics tools like BLAST or tools provided by your institution. Additionally, you can run a PCR with your primers and sequence the resulting PCR product to confirm that the correct DNA region has been amplified.


How can I add a restriction site to a primer while ensuring it does not interfere with the primer's function?

To add a restriction site to a primer without interfering with its function, you can place the site at the 5' or 3' end of the primer, away from the region that binds to the target DNA. This way, the restriction site will not disrupt the primer's ability to anneal to the target sequence during PCR or other molecular biology techniques.


How can I make a primer for PCR?

To make a primer for PCR, you will need to design a short piece of single-stranded DNA that is complementary to the target DNA sequence you want to amplify. This primer will serve as a starting point for the DNA polymerase to begin replicating the target sequence during the PCR process. You can design the primer using bioinformatics tools and order it from a supplier specializing in molecular biology reagents.


What is meant by primer needs to span exon-exon junction?

When a primer needs to span an exon-exon junction, it means that the primer sequence is designed to bind to regions on either side of an intron-exon boundary in order to amplify the spliced mRNA sequence. This helps ensure that the resulting PCR product only includes the mature mRNA sequence without any intervening intronic sequences.


When a rifle fires a sequence of events take place after the firing strikes the primer what is the next event in the sequence?

Primer being struck by firing pin causes fire which ignites the gunpowder which burns and produces gas which forces the projecitle down the bore of the weapon.


What is primer in pcr?

A primer (oligonucleotide of a specific sequence) is required for Taq polymerase to extend the template strand by adding complementary nucleotides. The function of the primer is to anneal to the template strand at a very specific site and facilitate the initiation of strand elongation mediated by Taqploymerase.


What sequence in human DNA is the forward primer complementary to?

forward primers are complementary to anti sense strand of the dsDNA


How can reverse primer design be optimized for efficient amplification in PCR experiments?

Reverse primer design for efficient amplification in PCR experiments can be optimized by ensuring the primer has a high melting temperature, is specific to the target sequence, and does not form secondary structures. Additionally, primer length, GC content, and avoiding primer-dimer formation are important factors to consider for successful PCR amplification.


How can one effectively design a primer for PCR?

To effectively design a primer for PCR, one should consider the following factors: the target DNA sequence, primer length (usually 18-22 nucleotides), GC content (around 50), absence of self-complementarity or secondary structures, and specificity to the target region. Additionally, primer design tools and software can aid in optimizing primer sequences for successful PCR amplification.