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What word names an ordered list of numbers?
A sequence.
What is the sequence 1 2 3 5 8 called?
Fibonacci sequence
What is the 21st sequence in this sequence 1 -2 -5 -8 -11?
26073.
What is this number sequence 2 3 5 7?
Prime number sequence.
What is 5 5 5 5 5 5 5 5 5 5 5 5 5 5?
555,555,555,555 That is a sequence of 14 number 5's. The Beatles once used a sequence of number 9's, in their rather strange song, Revolution # 9, on the White Album (which is actually named "The Beatles"). This sequence is presumably an homage to the Beatles.
What is the specific primer sequence used in the PCR amplification of the target gene?
The specific primer sequence used in the PCR amplification of the target gene is 5'-AGCTGATCGATCGATCGATCG-3'.
When a rifle fires a sequence of events takes place. After the firing pin strikes the primer what is the next event in the sequence?
When a rifle fires, a sequence of events takes place. After the firing pin strikes the primer, what is the next event in the sequence
When a rifle fires, a sequence of events takes place. After the firing pin strikes the primer, what is the next event in the sequence?
The primer explodes and ignites the gunpowder.
Nucleotide sequence that starts PCR processes?
Primer sequences
How can I check am I using correct primer sequence in my PCR?
You can check the correctness of your primer sequence by performing a basic sequence alignment using bioinformatics tools like BLAST or tools provided by your institution. Additionally, you can run a PCR with your primers and sequence the resulting PCR product to confirm that the correct DNA region has been amplified.
How can I add a restriction site to a primer while ensuring it does not interfere with the primer's function?
To add a restriction site to a primer without interfering with its function, you can place the site at the 5' or 3' end of the primer, away from the region that binds to the target DNA. This way, the restriction site will not disrupt the primer's ability to anneal to the target sequence during PCR or other molecular biology techniques.
What is the process for designing a reverse primer for a specific DNA sequence?
Designing a reverse primer for a specific DNA sequence involves identifying the complementary sequence on the opposite strand of the target DNA, determining the optimal length and composition of the primer, and ensuring it has the necessary characteristics for successful PCR amplification. This includes considering factors such as melting temperature, GC content, and avoiding self-complementarity or hairpin structures. The primer should also be specific to the target sequence to avoid non-specific amplification.
How can I make a primer for PCR?
To make a primer for PCR, you will need to design a short piece of single-stranded DNA that is complementary to the target DNA sequence you want to amplify. This primer will serve as a starting point for the DNA polymerase to begin replicating the target sequence during the PCR process. You can design the primer using bioinformatics tools and order it from a supplier specializing in molecular biology reagents.
What is meant by primer needs to span exon-exon junction?
When a primer needs to span an exon-exon junction, it means that the primer sequence is designed to bind to regions on either side of an intron-exon boundary in order to amplify the spliced mRNA sequence. This helps ensure that the resulting PCR product only includes the mature mRNA sequence without any intervening intronic sequences.
What does a primer do in PCR and how does it contribute to the amplification of DNA fragments?
A primer in PCR is a short piece of DNA that binds to a specific target sequence on the DNA template. It serves as a starting point for DNA synthesis by the DNA polymerase enzyme. The primer helps the enzyme to accurately copy the target DNA sequence, leading to the amplification of the DNA fragment during PCR.
When a rifle fires a sequence of events take place after the firing strikes the primer what is the next event in the sequence?
Primer being struck by firing pin causes fire which ignites the gunpowder which burns and produces gas which forces the projecitle down the bore of the weapon.
What is primer in pcr?
A primer (oligonucleotide of a specific sequence) is required for Taq polymerase to extend the template strand by adding complementary nucleotides. The function of the primer is to anneal to the template strand at a very specific site and facilitate the initiation of strand elongation mediated by Taqploymerase.
