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Q: What is the sequence of the primer 5' -ACGTGCT-3'?
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When a rifle fires a sequence of events takes place. After the firing pin strikes the primer what is the next event in the sequence?

When a rifle fires, a sequence of events takes place. After the firing pin strikes the primer, what is the next event in the sequence


When a rifle fires, a sequence of events takes place. After the firing pin strikes the primer, what is the next event in the sequence?

The primer explodes and ignites the gunpowder.


Nucleotide sequence that starts PCR processes?

Primer sequences


How can I check am I using correct primer sequence in my PCR?

You can check the correctness of your primer sequence by performing a basic sequence alignment using bioinformatics tools like BLAST or tools provided by your institution. Additionally, you can run a PCR with your primers and sequence the resulting PCR product to confirm that the correct DNA region has been amplified.


What is meant by primer needs to span exon-exon junction?

When a primer needs to span an exon-exon junction, it means that the primer sequence is designed to bind to regions on either side of an intron-exon boundary in order to amplify the spliced mRNA sequence. This helps ensure that the resulting PCR product only includes the mature mRNA sequence without any intervening intronic sequences.


When a rifle fires a sequence of events take place after the firing strikes the primer what is the next event in the sequence?

Primer being struck by firing pin causes fire which ignites the gunpowder which burns and produces gas which forces the projecitle down the bore of the weapon.


What is primer in pcr?

A primer (oligonucleotide of a specific sequence) is required for Taq polymerase to extend the template strand by adding complementary nucleotides. The function of the primer is to anneal to the template strand at a very specific site and facilitate the initiation of strand elongation mediated by Taqploymerase.


What sequence in human DNA is the forward primer complementary to?

forward primers are complementary to anti sense strand of the dsDNA


What is difference between DNA probe and primer?

A DNA probe is a single-stranded DNA sequence used to detect complementary sequences, whereas a primer is a short single-stranded DNA sequence used to initiate DNA synthesis during PCR. Probes are used to identify specific sequences in a sample, while primers are used to amplify a specific target sequence.


What are primer dimers in pcr?

Primer dimers are unintended products that form when the primers used in a PCR reaction bind to each other rather than to the target DNA sequence. This can result in inefficient amplification of the target sequence and lead to false results. To minimize primer dimers, design specific primers with appropriate melting temperatures and ensure optimal PCR conditions.


What does the first nucleotide attach to because DNA polymerase can only add nucleotides to a pre-existing strand?

A primer molecule is required for DNA polymerase to initiate the addition of nucleotides. This primer provides a starting point for DNA polymerase to begin adding nucleotides in the correct sequence. Once the primer is in place, DNA polymerase can add nucleotides complementary to the template strand.


How do you mixomni 2k primer?

to thin 2k primer or primer fillers such as dupont 1040r a raitio of 5:1 is used ie: 500mls primer to 100mls activator, if the mixture needs to be thinned, first mix the primer and activator to above ratio then add thinners at 20mls of thinners per 100mls primer mix. eg: 500mls primer +100mls activator= 600mls, 6x20mls=120mils of thinners for 600mils primer mix.