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Profit is calculated by subtracting costs from revenue.
Mark-up, it is not profit. Profit must account for other fixed costs associated with selling
You buy a coconut for 1 rupee. You sell that coconut to me for 2 rupees. You have made 1 rupee (minus your other costs) profit.
The subtrahend is subtracted from the minuend
2010 subtracted from1981 = 29