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Q: Why measure the ratio of 260 and 280 nm?
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What is the result after isolating DNA?

After isolating DNA, you have obtained a purified sample of DNA that can be used for various downstream applications such as PCR, DNA sequencing, genetic testing, and cloning. This purified DNA can provide valuable information about an organism's genetic makeup, structure, and function.


What does nm in calculation of ultraviolet rays mean?

In the context of ultraviolet rays, nm stands for nanometers, which is a unit of measurement used to describe the wavelength of these rays. Ultraviolet radiation is classified into different categories based on their respective wavelength ranges, such as UVA (320-400 nm), UVB (280-320 nm), and UVC (100-280 nm).


What is the typical wavelength of ultraviolet?

The typical wavelength of ultraviolet (UV) light ranges from 10 nm to 400 nm. UV light is further divided into UVA (315-400 nm), UVB (280-315 nm), and UVC (100-280 nm) based on its specific wavelength range.


Which of the peptides would absorb light at 280 nm?

Peptides containing aromatic amino acids such as tryptophan, tyrosine, and phenylalanine are known to absorb light at 280 nm. These amino acids are capable of absorbing UV light due to their aromatic side chains, with tryptophan having the highest molar absorptivity at 280 nm.


Range of wavelengths for ultraviolet rays?

Ultraviolet rays have wavelengths ranging from about 10 nm to 400 nm. They are categorized into different subtypes based on their wavelength: UVA (315-400 nm), UVB (280-315 nm), and UVC (100-280 nm).


What is wave length of ultra violet rediation?

The wavelength of ultraviolet radiation ranges from about 10 nm to 400 nm. Within this range, different types of UV radiation have varying wavelengths: UVA (315-400 nm), UVB (280-315 nm), and UVC (100-280 nm).


Which pair of amino acid will have the highest absorbance at 280 nm?

Aromatic amino acids such as tryptophan and tyrosine will have the highest absorbance at 280 nm due to their aromatic ring structures. These amino acids have strong UV absorbance properties and are commonly used in protein quantification assays due to their unique spectral properties at 280 nm.


What is the frequency range of uv radiation?

Ultraviolet (UV) radiation spans a wide range of frequencies, typically from about 10^15 to 10^17 Hz. This range is further divided into UVA (315-400 nm), UVB (280-315 nm), and UVC (100-280 nm) wavelengths.


Why uracil used in hplc calibration?

higly unretainable and has high absorption at 260 nm


What waves have wavelength between 400 nm and 10 nm?

Waves with wavelengths between 400 nm and 10 nm fall within the ultraviolet (UV) range of the electromagnetic spectrum. This range includes UVA, UVB, and UVC rays, with UVA having wavelengths between 315-400 nm, UVB between 280-315 nm, and UVC between 100-280 nm. These waves have increasingly higher energy and are known for their potential harmful effects on living organisms.


What is the wave length in centimeters of UV light?

The wavelength of UV light falls within the range of 10-400 nanometers, which is equivalent to 0.001-0.04 centimeters.UV light is categorized into UVA (315-400 nm), UVB (280-315 nm), and UVC (100-280 nm) based on their wavelengths.


How fast is 250 nm?

nm stands for nanometres, it is not a measure of speed, but length