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To make an 8-fold dilution, you would mix 1 part of the substance you want to dilute with 7 parts of the diluent (usually water or buffer solution). This results in a total of 8 parts, with 1 part being the original substance and 7 parts being the diluent. This dilution reduces the concentration of the original substance by a factor of 8.
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JudyAn 8-fold dilution means you are making your solution 1/8 less concentrated. So, if you want to make 100 ml of a solution diluted 8 fold you would do as follows:
1/8=.125 100*.125= 12.5
Mix 12.5 mL of your concentrated solution with 87.5 mL of your solvent (water etc..) to bring the final volume to 100 mL.
If you wanted to perform a ten fold dilution you would simply mix 10 mL of your concentrated solution with 90 mL of your solvent.
1/10=.1 100*.1=10
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