It is a mixture of 1 part of the active ingredient (by volume) to 10 parts of the solvent.
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To make an 8-fold dilution, you would mix 1 part of the substance you want to dilute with 7 parts of the diluent (usually water or buffer solution). This results in a total of 8 parts, with 1 part being the original substance and 7 parts being the diluent. This dilution reduces the concentration of the original substance by a factor of 8.
Assuming you mean 10/20, (10/20)/(4-5)=0.5/-1=-1/2 Assuming you mean 10.20, 10.2/-1=-10.2 Assuming you mean 10-20, (10-20)/(-1)=(-10)/-1=10
To make a 1 to 5 dilution you mix 1 part of your substance with 4 parts water. ie: Mix 1 tablespoon of creamer with 4 tables spoons of coffee, and the coffee is 1/5 creamer now.
1X PBS might mean 1 in 10 solution 1/10
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