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The Enterotube II System is generally used to distinguish between Enterobacteriaceae. Members of this family are generally glucose fermenters and some also produce gas. Since you get a "2" for glucose fermentation and a "1" for gas production, the value of the first number should be 2 or 3 in order to identify an organism using the Enterotube II System.

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Q: Why shoud the first digit in the enterotube II code always be equal to or greater than 2?
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