25 o because sOme of them are perpendicular
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None in the Roman alphabet.
The latin alphabet for English has 52 letters, if you count both upper case and lower case. Otherwise, there no other alphabet with 52 letters.
There are 26 letters in the English version of the Latin alphabet.
It depends on the font. Using this font, I count 8 upper case letters: E F H I M N W Z and 2 Lower case letters: w z
The letters A, K, M, N, R, V, W, Y, and Z all have lines in them that are not perpendicular.