Add 2 mL of culture to 20 mL of buffer. 2/20 = 1/10
1 part of solution A plus 99 parts solution B
what is dilution rate for glycos
take 1 ml, add 9 ml water.
The dilution rate for liquid sevin concentrate for lawns is 1 Tablespoon per gallon of water which covers 94 square feet
Add 2 mL of culture to 20 mL of buffer. 2/20 = 1/10
In chemistry and biology, the dilution factor is the total number of unit volumes in which the material is dissolved. As I understand it, the dilution refers to the dilution ratio. If you add 1 part of something to 4 parts of something else, the dilution ratio is 1 to 4. The dilution factor counts all the parts and expresses the same thing as 1 out of 5.
33,4ml
dilute it 1 in 5000. likely best done with a serial or step dilution
So you need a 1 in 5 dilution (5x dilution i.e. 0.5/0.1), so 100/5 is 20ml stock diluted to 100ml
To make a 1 to 5 dilution you mix 1 part of your substance with 4 parts water. ie: Mix 1 tablespoon of creamer with 4 tables spoons of coffee, and the coffee is 1/5 creamer now.
1 part of solution A plus 99 parts solution B
If accuracy is not a problem the most simple way to produce 20 ml of a 1:400 dilution is to take 1:400 of 20 ml (i.e. 0.05 ml) of the original solution and add 19.95 ml of liquid. As pipettes are the most precise close to their maximum uptake volume and micro-pipettes are inherently much less precise than pipettes for larger volumes I'd do a two-step dilution. 1. step: 1.000 ml of original solution with 1 ml pipette + 4 times 4.750 ml with 5 ml pipette 2. step: 1.000 ml of solution from step 1 + 4 times 4.750 ml with 5 ml pipette.
0.03125 ounces.
You dilute it 1:10, then you take 1 part of that solution and mix it with 9 parts of the diluent. That will make the 1:100 dilution you need, incl. prevention of pipette inaccuracy.
1:4
You add 9.09ml of stock solution to a volumetric and make it up to 1 litre to get a 110 dilution