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What is the difference between a qualitative and a quantitative assay?
Wiki User
∙ 9y ago
In assays, or medical tests, both qualitative and quantitative tests occur. The qualitative test identifies the presence or absence of the pathogen or toxin (or other molecule) being tested for, while the quantitative test hopes to measure how much of that particular substance is in the body.
Wiki User
∙ 9y ago
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What is a four point biological assay?
Four point assay refers to an estimate of the potency of a drug by measuring the response it produces in a biological it produces i n abiological preparation
How do you calculate the assay percent in chemicals?
4.4 g of NaOH
What are standard assay conditions?
A standard assay is an analytic procedure for measuring the presence or amount or activity of the analyte. The general steps are; sample processing, target specific identification, target amplification system, and detection. detection.
What is the formula to calculate percent assay?
Final outcome ÷ possible outcome X100 (eg. If I got 23 out of 30 for a test then ---> 23÷30 X100 = 76.66= 77% ) hope this helped... :]*
How do you make a standard curve graph?
Standard curves are used to determine the concentration of substances. First you perform an assay with various known concentrations of a substance you are trying to measure. The response might be optical density, luminescence, fluorescence, radioactivity or something else. Graph these data to make a standard curve - concentration on the X axis, and assay measurement on the Y axis. Also perform the same assay with your unknown samples. You want to know the concentration of the substance in each of these unknown samples. To analyze the data, fit a line or curve through the standards. For each unknown, read across the graph from the spot on the Y-axis that corresponds to the assay measurement of the unknown until you intersect the standard curve. Read down the graph until you intersect the X-axis. The concentration of substance in the unknown sample is the value on the X-axis. In the example below, the unknown sample had 1208 counts per minute, so the concentration of the hormone is 0.236 micromolar. Prism makes it very easy to fit your standard curve, and to read (interpolate) the concentration of unknown samples.
What is assay for tablets?
Assay is done for qualitative and quantitative estimation of the content and amount of substance in the product . the product can be either raw material or final product.
What is the difference between assay and analysis?
Assay is the quantitative determination of the amount of a component in a sample. Purity is the determination of the impurities in the sample. It can be quantitative, semi-quantitative or qualitative. For example : if you dissolve 4 grams of compound A (with no impurities in it) in 6 grams of water, you get a solution of compound A with 100% purity and 40% assay. From the ICH Guideline Q2A (Validation of Analytical methods) : "- Testing for impurities can be either a quantitative test or a limit test for the impurity in a sample. Either test is intended to accurately reflect the purity characteristics of the sample. [...] - Assay procedures are intended to measure the analyte present in a given sample. In the context of this document, the assay represents a quantitative measurement of the major component(s) in the drug substance."
What is the difference between primary and secondary screening of microorganisms?
primary screening is a solid agar plate assay method and is a qualitative method after which secondary screening is done,in which the microbes are screened in a liquid shake flask condition and assessed quantitatively.
What is difference between end point and kinetic assay in biochemistry?
No one knows
Difference between assay as is basis and dried basis?
assay on as is basis includes the weight of water, residual solvents, moisture absorbed, if any. but assay on dried basis will remove all athe above by drying the sample under analysis.
What is the difference between assay on as is basis and assay on anhydrous basis?
assay on as is basis includes the sample+water content+residual solvents+others. but assay on as is basis takes water content into account that means weight of the water present in the sample will be added in weighing the sample quantity of API required.
What is the difference between potency and assay?
Potency is the purity of the compound ;this states % available of the main component without water and residual solvents Assay is defined as Potency plus water and residual solvents and other remaining fractions please note assay can also be expressed as as is basis or dry basis Assay and Potency are same if assay is exprtessed as as is basis please note Potency word is mainly used for Antibiotics and bilogicals courtsey ; CDER , USFDA ; Dr.Uttam Krishna
What is difference between anhydrous and dried basis?
1. anhydrous means : Means remove the water content & solvent free value calculated in the assay. 2. On dry base : Means remove the loss on dry & solvent free value calculated in the assay
What is an assay pound?
An assay pound is a small standard weight used in assaying bullion, which varies between assayers, but is generally around half a gram.
What is an assay?
An assaying is a trial by assay, thorough examination and determination.
What is protein assay?
Protein assay is the determination of concentration or total level of protein in a solution.There are various protein assays employed like bradford assay and lowry assay
What is a bioassay?
Noun 1. bioassay - appraisal of the biological activity of a substance by testing its effect on an organism and comparing the result with some agreed standard bio-assayassay - a quantitative or qualitative test of a substance (especially an ore or a drug) to determine its components; frequently used to test for the presence or concentration of infectious agents or antibodies etc.pregnancy test - a physiological test to determine whether a woman is pregnant Verb 1.bioassay - subject to a bio-assay assay - analyze (chemical substances) As per the Online Dictionary.
