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How do you make a standard curve graph?
Standard curves are used to determine the concentration of substances. First you perform an assay with various known concentrations of a substance you are trying to measure. The response might be optical density, luminescence, fluorescence, radioactivity or something else. Graph these data to make a standard curve - concentration on the X axis, and assay measurement on the Y axis. Also perform the same assay with your unknown samples. You want to know the concentration of the substance in each of these unknown samples. To analyze the data, fit a line or curve through the standards. For each unknown, read across the graph from the spot on the Y-axis that corresponds to the assay measurement of the unknown until you intersect the standard curve. Read down the graph until you intersect the X-axis. The concentration of substance in the unknown sample is the value on the X-axis. In the example below, the unknown sample had 1208 counts per minute, so the concentration of the hormone is 0.236 micromolar. Prism makes it very easy to fit your standard curve, and to read (interpolate) the concentration of unknown samples.
What is a four point biological assay?
Four point assay refers to an estimate of the potency of a drug by measuring the response it produces in a biological it produces i n abiological preparation
How do you calculate the assay percent in chemicals?
4.4 g of NaOH
How do you convert scfh to acfh?
To convert standard cubic feet per hour (SCFH) to actual cubic feet per hour (ACFH), you need to account for the pressure and temperature conditions of the gas. The formula to convert is: [ ACFH = SCFH \times \frac{P_{standard}}{P_{actual}} \times \frac{T_{actual}}{T_{standard}} ] where ( P ) is pressure and ( T ) is temperature, with standard conditions typically being 14.7 psi and 60°F. Make sure to use consistent units for pressure and temperature.
Is mass measured in cubic centimeters?
No. Cubic centimeters are units of volume. Mass is measured in grams, and the standard mass is a kilogram of metal kept under the strictest conditions and tightest security in France.
