How to calculate BOD value in a sample?

Answer 1

if unseeded, BOD = (D1-D2)/P
if seeded, BOD = ((D1-D2)-(B1-B2)f)/P

D1 = DO of diluted seeded wastewater
D2 = DO of wastewater after incubation
B1 = DO of diluted seed sample
B2 = DO of seed sample after incubation
f = ratio of seed volume in seeded wastewater test to seed volume in BOD test on seed
P = decimal fraction of wastewater sample used. (vol. of wastewater)/(vol. of dilution water plus wastewater)

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