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sometimes it goes blank when your under pressure like for instence when you have a test
2 + 5 * 3 is one possible answer.
The Biochemical Oxygen Demand (BOD) of a sample is the amount of dissolved oxygen required by aerobic microorganisms to breakdown organic matter in the sample.The test is simple:Measure the Dissolved Oxygen content of the sample (a)Place in a sealed darkened bottleIncubate for 5 days at 20'CMeasure the Dissolved Oxygen content of the incubated sample (b)Generally you're using this test to analyse contaminated water, so you'll want to dilute it first - it may be that aren't enough aerobic microorganisms present to give you a measurable result, in which case you'll need to inoculate the sample with a suitable culture.The BOD of the sample = a - bBOD is expressed in mg O2/L of sample.Extra CreditGenerally you're using this test to analyse contaminated water, so you'll want to dilute it first - it may be that aren't enough aerobic microorganisms present to give you a measurable result, in which case you'll need to inoculate the sample with a suitable culture.The BOD of a diluted innoculated sample = ((a - b) - BOD of inoculated culture) * dilution factor
Reagent Blank : Take reagent and add deionised water (in place of sample to be tested). Now measure the OD at specific wavelength --> this OD is your reagent blank. Substract this OD from your test result (with sample) to avoid any false +ve effect due to colour of reagents itself.Sample Blank : Take sample and measure the OD without adding reagents --> this OD is your sample blank. Substract this OD from your test result to avoid any false +ve effect due to colour and turbidity of sample itself. As it is the fact that colour and turbidity of each sample would vary from one to another.So now it is clear that Reagent blank is used to avoid bias due to colour of reagents and Sample blank is used to avoid bias due to sample itself.
Four. An-y-bod-y