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The numerical apertures of the condenser and low power objective lenses are 1.25 and 0.25 you are supplied with a filter that selects a wavelength of 520nm what is the limit of resolution?
Wiki User
∙ 12y ago
346.7nm's
Wiki User
∙ 12y ago
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(2x)2 = 4 x2 Its numerical value depends on the value of 'x'.
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What is the limit of resolution if numerical aperture of condenser is 1.25 and low power objective lense is 25?
You also need to know the wavelength :)
What is the limited resolution formula?
D = wavelength / NA condensor + NA objective D being minimum distance at which two points can be resolved....... wave length of light used......condensor and objective are the numerical apertures of the condensor lens and objective lens
What influences resolution in regards to the compound binocular light microscope?
Resolution of a microscope is tied to the numerical aperture of the objective lens and the condenser but is influenced by other factors, such as alignment, type of specimen, wavelength of light, and contrast enhancing techniques. Read more: Define Resolution in Microscopes | eHow.com http://www.ehow.com/facts_5753341_define-resolution-microscopes.html#ixzz1kYyrj6D9
What is resolution of objective lens for 1.25 and 0.25 given wavelength of 520nm?
The formula for the resolution of an objective lens isR = (1.22(lamda))/(2*(NA))Where (lamda) is wavelength and (NA) is the numerical aperture
The wavelength of light used plus the numerical aperature governs?
To find resolution power of optical microscopes.
Why does resolution power not depend on numerical aperture in EM?
The minimum resolvable separation distance of a light microscope depends on the wavelength of illumination and the numerical aperature. Because the electron beam has a far smaller wavelength than light used in light microscopy, it achieves far better resolution and it doesn't even involve the NE.
Will you be able to distinguish two points that are 330 nm apart as being separate or will they blur into one?
If the numerical apertures of the condenser and low power objective lenses are 1.25 and 0.205 respectively and you are supplied with a filter that selects a wavelength of 521 nm then the answer is YES! 520/(1.25 + 0.25) = 347 nm and your two points in question are shorter in distance as they are only 330 nm apart.
Four assessments of the quality of the image being seen using a light microscope?
[1] Brightness - How light or dark is the image? Brightness is related to the illumination system and can be changed by changing the voltage to the lamp (rheostat) and adjusting the condenser and diaphragm/pinhole apertures. Brightness is also related to the numerical aperture of the objective lens (the larger the numerical aperture, the brighter the image).[2] Focus - Is the image blurry or well-defined? Focus is related to focal length and can be controlled with the focus knobs. The thickness of the cover glass on the specimen slide can also affect your ability to focus the image -- it can be too thick for the objective lens. The correct cover-glass thickness is written on the side of the objective lens.[3] Resolution - How close can two points in the image be before they are no longer seen as two separate points? Resolution is related to the numerical aperture of the objective lens (the higher the numerical aperture, the better the resolution) and the wavelength of light passing through the lens (the shorter the wavelength, the better the resolution).[4] Contrast - What is the difference in lighting between adjacent areas of the specimen? Contrast is related to the illumination system and can be adjusted by changing the intensity of the light and the diaphragm/pinhole aperture. Also, chemical stains applied to the specimen can enhance contrast.
List the four assessments of the quality of the image being seen using a light microscope?
[1] Brightness - How light or dark is the image? Brightness is related to the illumination system and can be changed by changing the voltage to the lamp (rheostat) and adjusting the condenser and diaphragm/pinhole apertures. Brightness is also related to the numerical aperture of the objective lens (the larger the numerical aperture, the brighter the image).[2] Focus - Is the image blurry or well-defined? Focus is related to focal length and can be controlled with the focus knobs. The thickness of the cover glass on the specimen slide can also affect your ability to focus the image -- it can be too thick for the objective lens. The correct cover-glass thickness is written on the side of the objective lens.[3] Resolution - How close can two points in the image be before they are no longer seen as two separate points? Resolution is related to the numerical aperture of the objective lens (the higher the numerical aperture, the better the resolution) and the wavelength of light passing through the lens (the shorter the wavelength, the better the resolution).[4] Contrast - What is the difference in lighting between adjacent areas of the specimen? Contrast is related to the illumination system and can be adjusted by changing the intensity of the light and the diaphragm/pinhole aperture. Also, chemical stains applied to the specimen can enhance contrast.FROM VLA hacker
Calculate the resolving power if the wavelength is 600 nm and the numerical apertures are 0 0.2 0.4 0.6 0.8 and 1.0?
Use the Equation, Resolving Power=lambda/2(Numerical Aperture). So, given the values for Numerical Aperture(NA): If NA=0, then R=0, NA=0.2, then R=1500, NA=0.4, then R=750, etc. Simply solve the equation substituting the provided Numerical Aperture (NA) values in.
What is Rayleigh criterion of resolution?
According to Rayleigh Criteria, resolution is determined by the wavelength of imaging light (λ) and numerical aperture (ΝΑ) of the projection lens. Thus resolution is given by the following equation. R = k1 λ/ NA Where k1 is the process parameter describing the difficulty of the process
What is the formula for microscopic resolution?
S = (0.61 X λ)/(I x sin(x)) where: S = Resolution λ = wavelength I = Refractive index sin(x) = maximum angle of light gathering Both I and sin(x) are constants for a given objective lens, there product is referred to as N.A. or "Numerical Aperature".
